We narrowed to 4,369 results for: AR

1. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 NLRP1 P1214R

   Plasmid

   #166822

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   P1214R

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NET97 pmRFP-N2 (641)

   Plasmid

   #61994

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   AADACL1 (NCEH1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pGEX-2T-TRR-C421-Y2404F

   Plasmid

   #182846

   Purpose

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. STARR-seq luciferase validation vector_mP_SV40

   Plasmid

   #99310

   Purpose

   Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   SV40 enhancer
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-zfIl34

   Plasmid

   #168112

   Purpose

   Express zebrafish Il34, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

   Insert

   Il34 (il34 Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. nuc-yHS1-M7A,H102A

   Plasmid

   #159168

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1-M7A,H102A
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mito-yHS1-M7A,H102A

   Plasmid

   #159165

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A,H102A
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. STARR-seq luciferase validation vector_ORI_IGF1R

   Plasmid

   #99306

   Purpose

   Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr15: 99439352 -99440791
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. STT3A pmRFP (663)

    Plasmid

    #61997

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLKO-sh-Myocardin

    Plasmid

    #100769

    Purpose

    Lentiviral expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-sh-Myocardin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS663-pMCSG7-AcrIIC4Hpa

    Plasmid

    #113440

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS664-pMCSG7-AcrIIC5Smu

    Plasmid

    #113441

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Flag-Polß(WT)--IRES-hyg

    Plasmid

    #128667

    Purpose

    mammalian expression of Flag-Polß(WT) protein with hygromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 NLRP1 M77T

    Plasmid

    #166818

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    M77T

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221 NLRP1 A54T

    Plasmid

    #166815

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A54T

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A

    Plasmid

    #166832

    Purpose

    Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDB.His.MBP.3C NLRP1 UPA WT

    Plasmid

    #164010

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK234_055

    Plasmid

    #123920

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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