We narrowed to 24,167 results for: p

1. pET26b_AT118-H-V5-His

   Plasmid

   #222850

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH86966::AtU6p::sgRNA:NbCysP6

   Plasmid

   #223217

   Purpose

   Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbCysP6 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6 promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET26b_AT118-A-V5-His

   Plasmid

   #222849

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-A
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

   Plasmid

   #217514

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

   Plasmid

   #217509

   Purpose

   KD Recombinase dependent conditional 1X ALFA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
   

   Use

   Tags

   ALFA tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-3UTR

   Plasmid

   #202550

   Purpose

   Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media

   Depositor

   Rostislav Skrabana

   Article

   Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.

   Type

   Empty backbone

   Use

   Tags

   ATG initiation codon followed by an IGKV3 signal …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199450

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

   Plasmid

   #199451

   Purpose

   CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

   Plasmid

   #199452

   Purpose

   CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKTop::sptse5-CT (1169-1229)

    Plasmid

    #192948

    Purpose

    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1229)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1229)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKTop::tse5-CT (1169-1300)

    Plasmid

    #192956

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1300)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1300)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKTop::tse5-CT (1169-1317)

    Plasmid

    #192957

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1317)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1317)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKTop::tse5-CT (1169-1269)

    Plasmid

    #192954

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1269) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1269)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1269)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKTop::tse5-CT (1169-1229)

    Plasmid

    #192953

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1229)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1229)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKTop::sptse5-CT (1169-1281)

    Plasmid

    #192950

    Purpose

    For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1281)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1281)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKTop::sptse5-CT (1169-1269)

    Plasmid

    #192949

    Purpose

    For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1269)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1269)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKTop::sptse5-CT (1169-1300)

    Plasmid

    #192951

    Purpose

    For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1300)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for sptse5 (1169-1300)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Chinchilla+N122D–insM

    Plasmid

    #191227

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed methionine at 122 and replaced asparagine…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Sandgrouse+R111Q–insD

    Plasmid

    #191229

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed aspartic acid at 121 and replaced arginin…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Donor_C_FH_VIR

    Plasmid

    #186660

    Purpose

    Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge