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We narrowed to 59,686 results for: plasmid

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Showing: 1621 - 1640 of 59686 results

  3. pKMDDV_Plasmid_1

    Plasmid
    #249037
    Purpose
    Plasmid 1 containing constitutively expressed dCas9 and RBP-AD along with inducible scRNA_RFP
    Depositor
    Domitilla Del Vecchio
    Publication
    Aravind et al Cell Systems, 2026; 17
    Inserts
    dCas9
    MCP-SoxS(R93A/S101A)
    scRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    BBa_J23107, BBa_J23116, and PphlF
    Available since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    249037-plasmid-map-sequence-id-497730

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  5. pKMDDV_Plasmid_2b

    Plasmid
    #249057
    Purpose
    Plasmid 2b containing RFP with J306 scRNA binding site, GFP with J108 scRNA binding site and a constitutively expressed scRNA_GFP
    Depositor
    Domitilla Del Vecchio
    Publication
    Aravind et al Cell Systems, 2026; 17
    Inserts
    scRNA
    Fast Folding Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    BBa_J23117 and ptrc
    Available since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    249057-plasmid-map-sequence-id-511261

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  7. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  9. BcLOV4 fusion tools screening plasmid #1

    Plasmid
    #174511
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174511-plasmid-map-sequence-id-357205

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  12. CRISPR plasmid

    Plasmid
    #236553
    Purpose
    Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Cas9
    Expression
    Mammalian
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236553-plasmid-map-sequence-id-469881

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  15. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid
    #127637
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Publication
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    YPet
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PP7 coat protein (PCP)
    Expression
    Bacterial
    Promoter
    pJ21306
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127637-plasmid-map-sequence-id-251705

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  16. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid
    #247258
    Purpose
    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI
    Depositor
    Patrick Hsu
    Publication
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    Expression
    Mammalian
    Available since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247258-plasmid-map-sequence-id-490391

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  17. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

    Plasmid
    #101177
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101177-plasmid-map-sequence-id-194000

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  18. pcDNA3.1-IL2sp-6His-Asprosin plasmid

    Plasmid
    #190683
    Purpose
    Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.
    Depositor
    Atul Chopra
    Publication
    Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IL2-6xHis-Asprosin
    Tags
    IL2 signal peptide, 6xHis
    Expression
    Mammalian
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190683-plasmid-map-sequence-id-379339

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  20. pMH-HA/Joseph2-Multitagged Plasmid

    Plasmid
    #226726
    Purpose
    Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.
    Depositor
    Richard Cripps
    Publication
    Robinson et al Biotechniques. 2024;76(7):299-309. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pMH-HA/Joseph2
    Tags
    HA, Myc, GFP, Flag, V5, His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 24, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226726-plasmid-map-sequence-id-450280

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Showing: 1621 - 1640 of 59686 results