161,873 results

1. HA Display H3_A/Hong Kong/1/1968

   Plasmid

   #206506

   Purpose

   Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells

   Depositor

   IIya Finkelstein

   Insert

   H3_A/Hong Kong/1/1968
   

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   deleted amino acids 1651 to 1735

   Promoter

   CMV

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.82(hRluc/Puro)

   Plasmid

   #212933

   Purpose

   Renilla luciferase vector for investigating regions controlling transcription

   Depositor

   Pete Stecha

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DIO-EF1α-mKate2-biPAC

   Plasmid

   #169127

   Purpose

   AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Insert

   mKate2-biPAC
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   codon optimized to mouse

   Promoter

   EF1a

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-roxSTOProx-ZsGreen

   Plasmid

   #51274

   Purpose

   Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   roxSTOProx-ZsGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pFG2

   Plasmid

   #87250

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Use

   Tags

   parS segments

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD425

   Plasmid

   #202078

   Purpose

   Frame +0 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame +0 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-Mfn2

   Plasmid

   #141156

   Purpose

   Mammalian expression and labeling mitofusin 2

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   Mitofusin 2 (MFN2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBR_Cas9_sp1

    Plasmid

    #154851

    Purpose

    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

    Depositor

    John van der Oost

    Article

    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

    Inserts

    Spcas9 gene
    sgRNA scaffold
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Mutation

    Codon harmonized variant for expression in Rhodob…

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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