159,238 results

1. pBiT3.1-C_GEMIN5:1-1508

   Plasmid

   #211164

   Purpose

   Mammalian expression of full-length human GEMIN5. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   Gemin5:M1-M1508 (GEMIN5 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   R682Q natural variant (VAR_033807)

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. phage UbiC scAB-GFP

   Plasmid

   #104998

   Purpose

   Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   single chain Antibody against GCN4
   

   Use

   Lentiviral

   Tags

   GB1, HA tag, and sfGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-mCh-Cry2WT

   Plasmid

   #101221

   Purpose

   Blue-light dependent optogenetic protein Cry2

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2PHR
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME Integrin beta 1b

   Plasmid

   #105976

   Purpose

   entry clone for gateway cloning, Full length Integrin beta 1b without stop

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Integrinbeta1b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_CMV_FR-GECO1c

   Plasmid

   #224358

   Purpose

   Expresses red fluorescent calcium sensor FR-GECO1c

   Depositor

   Adam Cohen

   Article

   Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

   Insert

   FR-GECO1c
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mPA-GFP-Tubulin-C-18

   Plasmid

   #57160

   Purpose

   Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. DsRed2-Peroxisomes-4

   Plasmid

   #54503

   Purpose

   Localization: Peroxisomes, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   DsRed2 and Peroximal Targeting Signal 1 (PTS1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGL3 RARE-d2EGFP

    Plasmid

    #183055

    Purpose

    Destabilized EGFP fluorescent reporter for Retinoic Acid activity

    Depositor

    Chaya Kalcheim

    Article

    Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

    Insert

    d2EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    RARE, SV40

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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