164,577 results

1. pNeg-Ma-barnase-TAG3-TAG45

   Plasmid

   #197572

   Purpose

   Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Barnase - 2xTAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   3TAG and 45TAG

   Promoter

   araC and lpp

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 7TGP

   Plasmid

   #24305

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Promoter

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

   Plasmid

   #200729

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPQ

   Depositor

   Claudio Alarcón

   Insert

   HNRNPQ (SYNCRIP Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SlugCas9

   Plasmid

   #163793

   Purpose

   Expresses SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-fDIO-oG-WPRE-SV40pA

   Plasmid

   #74291

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   CAG

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBoBi-hTRPV4-C-GC6s

   Plasmid

   #154150

   Purpose

   To detect the calcium ion releases from TRPV4 channel

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   TRPV4 and GCamp6s (TRPV4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmCherry-N1-cofilin

   Plasmid

   #186750

   Purpose

   Expresses wildtype mCherry-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

   Plasmid

   #228902

   Purpose

   For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

   Depositor

   Bryan Roth

   Article

   Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

   Has Service

   AAV8

   Insert

   hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
   

   Use

   AAV and Synthetic Biology

   Tags

   HA and P2A mCitrine

   Expression

   Mammalian

   Mutation

   R108K

   Promoter

   Chicken Beta Actin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CL20_mEGFP_EEF2_SMARCA4

   Plasmid

   #205808

   Purpose

   Express mEGFP-tagged fusion protein, EEF2_SMARCA4 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EEF2_SMARCA4 (EEF2, SMARCA4 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid

    #117699

    Purpose

    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    HA tag, Myc-NES, and NLS

    Expression

    Mammalian

    Mutation

    codon optimized for murine expression

    Promoter

    None, 2A peptide from porcine teschovirus-1 polyp…

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge