4,180 results

1. FUGW-PK-hLC3

   Plasmid

   #61460

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-10His-mStayGold (E138D)

   Plasmid

   #211363

   Purpose

   Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Tags

   10xHis-tag

   Expression

   Mammalian

   Mutation

   E138D

   Promoter

   CMV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas

   Plasmid

   #62225

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgR

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Has Service

   Cloning Grade DNA

   Insert

   cas9
   

   Use

   CRISPR; Lambda red recombinase

   Expression

   Bacterial

   Promoter

   native cas9 promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR_SFFV

   Plasmid

   #79121

   Purpose

   Lentiviral vector for constitutive expression of genes (SFFV promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EZ-Tet-pLKO-Blast

   Plasmid

   #85973

   Purpose

   Tet/Dox inducible shRNA lentivirus with blasticidin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Promoter

   H1 / TetO

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLB

   Plasmid

   #11619

   Purpose

   pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

   Depositor

   Stephan Kissler

   Article

   Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV-U6+27-Tornado-Broccoli

   Plasmid

   #124360

   Purpose

   Overexpression of circular RNA (Broccoli)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-Broccoli
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME pHluorin2

   Plasmid

   #73794

   Purpose

   ratiometric pHluorin2 sequence in a middle entry clone

   Depositor

   David Raible

   Article

   Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

   Insert

   ratiometric pHluorin2
   

   Use

   Middle entry clone

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCMV-EcoEnv

    Plasmid

    #15802

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    MLV env (ecotropic)
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge