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Rab1ab cds in pGem-T Easy

Plasmid

#86218

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

Rab1ab (rab1ab Zebrafish)

Use

In situ probe/other

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMEtmem88aP1gfp

Plasmid

#90132

Purpose

Gateway Mid-entry clone containing promoter sequence specific for tmem88a driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aP1Egfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;yrkP1Egfp

Plasmid

#90168

Purpose

contains yrk specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkP1Egfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;snx5P1Egfp

Plasmid

#90166

Purpose

contains snx5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;tmem88aP1Egfp

Plasmid

#90167

Purpose

contains tmem88a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aP1Egfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;sheCE1-P1Egfp

Plasmid

#90150

Purpose

contains She enhancer sequence, and She specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

sheE1P1Egfp (she Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;nrp1bCE1-P1Egfp

Plasmid

#90149

Purpose

contains nrp1b enhancer sequence, and nrp1b specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

nrp1bE1P1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;sheCE1-basEgfp

Plasmid

#90139

Purpose

contains She enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

sheE1basEgfp (she Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;tmem88aCE1-basEgfp

Plasmid

#90135

Purpose

contains tmem88a enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1basEgfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;nrp1bCE1-basEgfp

Plasmid

#90134

Purpose

contains nrp1b enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

nrp1bE1basEgfp

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMEyrkP1gfp

Plasmid

#90133

Purpose

Gateway Mid-entry clone containing promoter sequence specific for yrk driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkP1Egfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMEcldn5bP1gfp

Plasmid

#90124

Purpose

Gateway Mid-entry clone containing promoter sequence specific for cldn5b driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bP1Egfp (cldn5b Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5EyrkE1

Plasmid

#90119

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of yrk

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkE1 (yrk Zebrafish)

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5Etmem88aE1

Plasmid

#90112

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of tmem88a

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1 (tmem88a Zebrafish)

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX1ZFCNGA5

Plasmid

#69859

Purpose

GST tagged protein for antibody production screening

Depositor

Gary Matthews

Article

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

GST

Expression

Bacterial

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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Hox B7a

Plasmid

#36861

Depositor

Robert Ho

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hox B7a (hoxb7a Zebrafish)

Use

Riboprobe synthesis

Expression

Bacterial

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
Hox B8a

Plasmid

#36862

Depositor

Robert Ho

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hox B8a (hoxb8a Zebrafish)

Use

Riboprobe synthesis

Expression

Bacterial

Mutation

D238E

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pBS ZfHoxb9a

Plasmid

#27529

Depositor

Victoria Prince

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxb9a (hoxb9a Zebrafish)

Use

Cloning vector

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pGEM-T ZfHoxc8a

Plasmid

#27538

Depositor

Victoria Prince

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxc8a (hoxc8a Zebrafish)

Expression

Bacterial

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
M50 Super 8x TOPFlash

Plasmid

#12456

Purpose

Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2pA2-mnx1:MLS-KillerRed

Plasmid

#115515

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway Cloning

Tags

MLS

Promoter

-3mnx1

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DD

Plasmid

#37275

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

Depositor

David Grunwald

Article

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

Flag, Fok I DD, Tal-C, and Tal-N

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Acvr1ba-P2A-Acvr2ba

Plasmid

#232640

Purpose

Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

Depositor

Katherine Rogers

Article

Iannucci et al bioRxiv [Preprint]. 2025 Apr 19:2025.04.

Insert

Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSN001 pME minprom_mCerulean

Plasmid

#195959

Purpose

Gateway pME fluorophore

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter mCerulean

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-myr-tdTomato-SV40pA-FKF

Plasmid

#119867

Purpose

Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Tags

2x myristoylation

Expression

Bacterial

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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E. coli 16S toehold switch sensor

Plasmid

#110698

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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