We narrowed to 11,416 results for: 112

1. pDONR223_IDH1_WT

   Plasmid

   #82133

   Purpose

   Gateway Donor vector containing IDH1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

   Plasmid

   #58492

   Purpose

   retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

   Depositor

   David Ron

   Article

   Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

   Insert

   IRE1a (ERN1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and preprotrypsin signal sequence

   Expression

   Mammalian

   Mutation

   deleted amino acids P29 to D408 and bearing mutat…

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Anti-Ankyrin-G (staining) [N106/43R]

   Plasmid

   #128635

   Purpose

   Mammalian Expression Plasmid of anti-Ankyrin-G (staining) (Human). Derived from hybridoma N106/43.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Ankyrin-G (staining) (Human) recombinant mouse monoclonal antibody (ANK3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

   Plasmid

   #78174

   Purpose

   AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   human synapsin

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

   Plasmid

   #126477

   Purpose

   Expresses Cas9-5xFlag-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   5xFlag
   TdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. px330-Pten-2

   Plasmid

   #162552

   Purpose

   sgRNA targeting Pten

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT/TO-V5-SHLD3

   Plasmid

   #114124

   Purpose

   Expresses N-terminally tagged V5-SHLD3 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pETM30-ARC105

   Plasmid

   #133426

   Purpose

   Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   PCQAP (MED15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains amino acids 5-78 fused to C-terminal of …

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221-FOXQ1

   Plasmid

   #101628

   Purpose

   Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   FOXQ1 (FOXQ1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DDR1 gRNA (BRDN0001162484)

    Plasmid

    #78011

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DDR1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DDR1 (DDR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DDR1 gRNA (BRDN0001162509)

    Plasmid

    #78012

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DDR1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DDR1 (DDR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG-KIF5A tail only

    Plasmid

    #166956

    Purpose

    Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (820-1027aa)

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221_MFSD12

    Plasmid

    #131940

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153538

    Purpose

    AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    EF1α1.1

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSN879

    Plasmid

    #190693

    Purpose

    Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

    Depositor

    Shinsuke Niwa

    Article

    Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

    Insert

    mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-IDH1-R132L

    Plasmid

    #116411

    Purpose

    Lentiviral expression of IDH1 R132L

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R132L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B52 + CHEK2 sgSTOP

    Plasmid

    #100712

    Purpose

    B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

    Plasmid

    #204697

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    direct fusion of BRD4 to p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-AMIGO3-COMP5AP-AviTag-9xHis

    Plasmid

    #157369

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    AMIGO3 (AMIGO3 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pD649-HAsp-AMIGO3-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156805

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    AMIGO3 (AMIGO3 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-2TK-arc105

    Plasmid

    #133425

    Purpose

    Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-95 or ARC105 fused to the …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti/TO/EYFP-P2A-IDH1(HA)

    Plasmid

    #122482

    Purpose

    A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

    Depositor

    Eric Huang

    Article

    Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

    Insert

    isocitrate dehydrogenase 1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    HA and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
    

    Use

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ankyrin-G scFv [N106/20]

    Plasmid

    #190525

    Purpose

    Mammalian Expression of Ankyrin-G scFV. Derived from hybridoma N106/20.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Ankyrin-G (Homo sapiens) recombinant scFV (ANK3 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Anti-Ankyrin-G [N106/36]

    Plasmid

    #190297

    Purpose

    Mammalian Expression Plasmid of anti-Ankyrin-G (Human). Derived from hybridoma N106/36.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Ankyrin-G (Homo sapiens) recombinant Mouse monoclonal antibody (ANK3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-IDH1-P33S

    Plasmid

    #116409

    Purpose

    Lentiviral expression of IDH1 P33S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    P33S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMIG-AVITEV- hNFATc1/aA

    Plasmid

    #74057

    Purpose

    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform alpha-A (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    mutation G1157A [R235Q] and the silent mutation C…

    Promoter

    pMSCV-LTRs

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWZL Neo Myr Flag PNKP

    Plasmid

    #20594

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PNKP (PNKP Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pEGFP-ARAP3(R307,8A)

    Plasmid

    #39486

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3(R307,8A) (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R307,8A = PH domain mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pLV-EF1a-KIF5A-HA-IRES-Puro

    Plasmid

    #166952

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR223-TP53RK

    Plasmid

    #23774

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TP53RK (TP53RK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

33. Drd1a->M71-IRES-tauGFP ACNF TV

    Plasmid

    #105073

    Purpose

    Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette

    Depositor

    Peter Mombaerts

    Article

    Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

    Insert

    Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Drd1a coding sequence followed by IRES-tauGFP ACNF

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. px330-Apob-2

    Plasmid

    #162549

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. px330-Apob-1

    Plasmid

    #162548

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. px330-Apob-3

    Plasmid

    #162550

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. px330-Apob-4

    Plasmid

    #162551

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-ARAP3(C504A)

    Plasmid

    #39485

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3(C504A) (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C504A = Arf GAP domain mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pT/CAGGS-mVenus-P2A-NRASG12V

    Plasmid

    #236072

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    CAGGS

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT/UBC-mVenus-P2A-NRASG12V

    Plasmid

    #236074

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Ubc

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pT/CAGGS-NRASG12V/D38A-IRES-mVenus

    Plasmid

    #236077

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V D38A (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V D38A

    Promoter

    CAGGS

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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42. pT/CAGGS-NRASG12V-IRES-mVenus

    Plasmid

    #236076

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    CAGGS

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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43. pT/PGK-mVenus-P2A-NRASG12V

    Plasmid

    #236073

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    PGK

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. pcDNA3.1-DDR1-F866Y-V5/HIS

    Plasmid

    #236009

    Purpose

    expression of the F866Y kinase defective mutant variant of human DDR1 that might be associated with lung Adenocarcinoma

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human DDR1-F866Y receptor tyrosine kinase, full length (DDR1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    F866Y substitution

    Promoter

    CMV

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28b-Hf-RfxCas13d-His

    Plasmid

    #234480

    Purpose

    Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed four alanine to valine in positions 134, …

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)

    Plasmid

    #204701

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300(deltaI) (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of intrinsically disordered regions of p…

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

    Plasmid

    #204700

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300(I) (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of all TF-bidning domains and HAT in p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

    Plasmid

    #204699

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of all TF-bidning domains in p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)

    Plasmid

    #204698

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300(H*IT) (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    D1399Y substitution in p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pD649-HAsp-LRRC4C-COMP5AP-AviTag-9xHis

    Plasmid

    #157441

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LRRC4C (LRRC4C Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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