We narrowed to 6,480 results for: poly

1. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

   Plasmid

   #221082

   Purpose

   Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfGFP[r5M]_ACC-mEBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   CMV, T7

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPicZ:mTREK-1_cryst_I110D

   Plasmid

   #209640

   Purpose

   P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

   Depositor

   Dan Minor

   Article

   Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
   

   Use

   Tags

   SNS- 3C_cleavage_site-TAAA-GFP

   Expression

   Yeast

   Mutation

   aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA269

   Plasmid

   #216073

   Purpose

   Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

   Plasmid

   #212809

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(63)
   

   Use

   Tags

   NUbo and mCherry-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

   Plasmid

   #212804

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

   Plasmid

   #212803

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48)
   

   Use

   Tags

   NUbo and mCherry-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIp128-TET-NUbo-E3(48)-NLS-VSV

   Plasmid

   #212746

   Purpose

   Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48)
   

   Use

   Integrative vector

   Tags

   NLS-VSV and NUbo

   Expression

   Yeast

   Mutation

   Promoter

   Tet

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YIp128-TET-NUbo-E3(63)-NLS-VSV

   Plasmid

   #212756

   Purpose

   Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(63)
   

   Use

   Integrative vector

   Tags

   NLS-VSV and NUbo

   Expression

   Yeast

   Mutation

   Promoter

   Tet

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

   Plasmid

   #212800

   Purpose

   Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Tags

   CUbo and Myc

   Expression

   Mammalian

   Mutation

   HOIP(C885A)

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)

    Plasmid

    #212814

    Purpose

    Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and CUbo(K63R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT-TO-NUbo-mCherry-FLAG

    Plasmid

    #212798

    Purpose

    Constitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo
    

    Use

    Tags

    mCherry-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SUMO-Twinkle 43-684

    Plasmid

    #205056

    Purpose

    TWNK protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    TWNK (TWNK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks amino acid from 1-42

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIp128-CUP1-His7-NUbo-GFP

    Plasmid

    #212791

    Purpose

    Copper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His7-NUbo-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp128-CUP1-His6-CUbo-GFP

    Plasmid

    #212792

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIp128-CUP1-CUbo-AID*-GFP

    Plasmid

    #212796

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo-AID*-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    CUbo-AID*-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YIp211-ADH-NUbo-E3(63)-mCherry-VSV

    Plasmid

    #212759

    Purpose

    Constitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NUbo and mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YIp128-ADH-NUbo-E3(63)-mCherry-VSV

    Plasmid

    #212758

    Purpose

    Constitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NUbo and mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIp211-CUP1-NUbo-E3(63) I227A-VSV

    Plasmid

    #212757

    Purpose

    Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63) I227A
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Pib1(I227A)

    Promoter

    CUP1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YIp128-ADH-NUbo-E3(63)-NLS-VSV

    Plasmid

    #212755

    Purpose

    Constitutive expression of NUbo-E3(63)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIp211-ADH-NUbo-E3(48)-mCherry-VSV

    Plasmid

    #212749

    Purpose

    Constitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NUbo and mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YIp128-ADH-NUbo-E3(48)-mCherry-VSV

    Plasmid

    #212748

    Purpose

    Constitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NUbo and mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YIp128-ADH-NUbo-E3(48)-NLS-VSV

    Plasmid

    #212745

    Purpose

    Constitutive expression of NUbo-E3(48)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

    Plasmid

    #212740

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-mCherry-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

    Plasmid

    #212739

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-mCherry-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

    Plasmid

    #212737

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-NLS-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YIp211-ADH-myc-E3(1)-CUbo-VSV

    Plasmid

    #212736

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YIp128-ADH-myc-E3(1)-CUbo-VSV

    Plasmid

    #212735

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YIp128-ADH-CUbo-mCherry-VSV

    Plasmid

    #212734

    Purpose

    Consitutive expression of CUbo-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    CUbo
    

    Use

    Integrative vector

    Tags

    mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YIp128-ADH-NUbo-mCherry-VSV

    Plasmid

    #212731

    Purpose

    Consitutive expression of NUbo-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo
    

    Use

    Integrative vector

    Tags

    mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-NUbo-H2B-His8

    Plasmid

    #212810

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and NUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA5-FRT-TO-His6-NUbo-H2B-His8

    Plasmid

    #212811

    Purpose

    Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    6xHis-NUbo and 8xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5-FRT-TO-His8-H2B-CUbo

    Plasmid

    #212812

    Purpose

    Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and CUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

    Plasmid

    #212813

    Purpose

    Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and CUbo(K48R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SUMO-Twinkle 360-684

    Plasmid

    #205055

    Purpose

    TWNK protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    TWNK (TWNK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N terminal amino acid from 1-359

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV6-AN-DDK_V222A RAD23B

    Plasmid

    #201580

    Purpose

    Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation V222A

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV6-AN-DDK_F400A RAD23B

    Plasmid

    #201581

    Purpose

    Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation F400A

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-TRE-IEo

    Plasmid

    #201992

    Purpose

    AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Tight TRE

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. DsRed -7e

    Plasmid

    #191765

    Purpose

    Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Use

    Tags

    N terminal fusion of negatively charged polypetod…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. H1.M10.luc

    Plasmid

    #193706

    Purpose

    Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV.CBA.YFP.miR-E_shSrpk3-1

    Plasmid

    #180400

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-1 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AAV.CBA.YFP.miR-E_shSrpk3-2

    Plasmid

    #180401

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-2 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV.CBA.YFP.miR-E_shSrpk3-3

    Plasmid

    #180402

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-3 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pST_YlHD (pBS0275)

    Plasmid

    #185153

    Purpose

    For the mammalian expression of the Yarrowia fungus protein YlHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    YlHD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CDS12_Cas9-NLS

    Plasmid

    #136122

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_AtcoCas9-NLS(noStop)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pNG1304

    Plasmid

    #162488

    Purpose

    Expresses HelD K239A from Bacillus subtillis

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    HelD (helD Bacillus subtillis 168)
    

    Use

    Tags

    6His-V5

    Expression

    Bacterial

    Mutation

    Changed Lysine 239 to Alanine (K239A)

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge