We narrowed to 2,781 results for: ALC

1. pAAV-4mtD3cpv

   Plasmid

   #181874

   Purpose

   Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   4mtD3cpv
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   the 4mtD3cpv CDS differs from the recorded GeneBa…

   Promoter

   CaMKIIa

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_Xph15-GCaMP6f

   Plasmid

   #135538

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-L713F

   Plasmid

   #174794

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLS2-YFPc

   Plasmid

   #87166

   Purpose

   fluorescence complementation assay

   Depositor

   Silke Robatzek

   Article

   Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

   Insert

   FLS2 (FLS2 Mustard Weed)
   

   Use

   Tags

   YFPc

   Expression

   Mutation

   Promoter

   35s

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLS2-YFPn

   Plasmid

   #87165

   Purpose

   fluorescence complementation assay

   Depositor

   Silke Robatzek

   Article

   Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

   Insert

   FLS2 (FLS2 Mustard Weed)
   

   Use

   Tags

   YFPn

   Expression

   Mutation

   Promoter

   35s

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-MICU1-mEF

   Plasmid

   #31765

   Depositor

   Vamsi Mootha

   Article

   Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

   Insert

   MICU1 (MICU1 Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Mutated in both EF-hand domains: D231A, E242K, D4…

   Promoter

   Available since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-TetON-mEGFP-MYOD1_AroPERFECT

   Plasmid

   #215620

   Purpose

   For integration of MYOD1 AroPERFECT T2A mEGFP

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   MYOD1 AroPERFECT (MYOD1 Human)
   

   Use

   Tags

   T2A mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hPPP3CB-FLAG

    Plasmid

    #179135

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. GlycophorinA-CD4d3+4-bio

    Plasmid

    #73093

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinA with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin A (GYPA Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lactotransferrin-CD4d3+4-bio

    Plasmid

    #73124

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Lactotransferrin (LTF Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. ICAM4-CD4d3+4-bio

    Plasmid

    #73102

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ICAM4 (ICAM4 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-hSTING-dCTT

    Plasmid

    #214164

    Purpose

    Retroviral transduction of STING

    Depositor

    Nan Yan

    Article

    Wu et al J Exp Med. 2019 Apr 1;216(4):867-883. do

    Insert

    STING (dCTT) (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    dCTT

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CD55-CD4d3+4-bio

    Plasmid

    #73116

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CD55 (CD55 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ENDOD1-CD4d3+4-bio

    Plasmid

    #73114

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ENDOD1 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ENDOD1 (ENDOD1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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