We narrowed to 32,952 results for: uros

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pLKO.1-puro-irf3-shrna5

Plasmid

#127649

Purpose

Knock-down of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 shRNA (IRF3 Human)

Use

Lentiviral

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-UBE3B-BirA-Puro

Plasmid

#107352

Purpose

Expresses UBE3B fused to BirA in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B-BirA (UBE3B Human)

Use

Lentiviral

Tags

Biotin ligase

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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FUW-RLuc-T2A-PuroR

Plasmid

#102320

Purpose

expression of r-luciferase

Depositor

Rudolf Jaenisch

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Renilla luciferase

Use

Lentiviral

Promoter

Ubc

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29.4R]

Plasmid

#114518

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat). Derived from hybridoma N110/29.4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAuroraA-GFP-AURKA-mCherry

Plasmid

#99878

Purpose

Expression of GFP-AURKA-mCherry in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP and mCherry

Expression

Mammalian

Promoter

AURKA minimal

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPBT/EFS-Cas9-PuroTk

Plasmid

#170542

Purpose

Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

PuroTK

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-SUMO2nc-PURO

Plasmid

#208043

Purpose

Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

SUMO2nc (SUMO2 Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

Q90P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSIRIP-puro shRNA p19-2

Plasmid

#14090

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA -2 (Cdkn2a Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pSIRIP-puro shRNA p19-1

Plasmid

#14089

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA - 1 (Cdkn2a Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-IRF1

Plasmid

#117156

Purpose

Target site TTGCTCTTAGCATCTCGGCTG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pUC118-FLIP-FlpE-Puro

Plasmid

#84539

Purpose

a mock vector for generating FLIP-FlpE targeting vectors

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

FLIP-FlpE - Puro

Use

Cre/Lox and Mouse Targeting

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3B-IRES-Puro

Plasmid

#107345

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-puro GFP-p62 dC

Plasmid

#38282

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Truncate mutant 1-265 amino acids residues

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

Plasmid

#218529

Purpose

sgRNA targeting human EIF2AK1/HRI

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK1 (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Puro-Ak-ACE2

Plasmid

#154985

Purpose

Lentiviral vector expressing Human ACE2

Depositor

Sonja Best

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE Tight

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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