We narrowed to 4,132 results for: pCMV mammalian

1. SpyCas9 PE2*-3HA

   Plasmid

   #169854

   Purpose

   Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-star-M-MLV-3HA
   

   Tags

   3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-SUMO-A'-l4-G'-IRES-EmGFP

   Plasmid

   #209127

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'-IRES-EmGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-SUMO-A'-l4-G-IRES-EmGFP

   Plasmid

   #209126

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G-IRES-EmGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTBL649 CHYRON2 integration construct

   Plasmid

   #126446

   Purpose

   To integrate the CHYRON2 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON2 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YEE-NG-CBE

   Plasmid

   #227418

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTBL405 CHYRON1 integration construct

   Plasmid

   #126442

   Purpose

   To integrate the CHYRON1 locus at HEK293site3.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON1 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mito NAD+ biosensor

   Plasmid

   #186791

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   mito NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. cytoplasmic NAD+ biosensor

   Plasmid

   #186787

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. nuclear NAD+ biosensor

   Plasmid

   #186789

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

    Plasmid

    #209296

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 2.2

    Plasmid

    #34885

    Depositor

    Irvin Chen

    Article

    Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

    Insert

    Sindbis virus envelope protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    SGN UTR in E1 domain

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCiPS-NIFV

    Plasmid

    #110807

    Purpose

    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

    Depositor

    David Russell

    Article

    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

    Insert

    Pol
    

    Expression

    Mammalian

    Mutation

    2 mutations in the DDE catalyic core motif of the…

    Promoter

    CMV

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag Snail WT

    Plasmid

    #16218

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 2XMyc-LRRK2-3XKD

    Plasmid

    #25366

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

    Plasmid

    #128608

    Purpose

    Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

    Plasmid

    #128607

    Purpose

    Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag Snail 6SA

    Plasmid

    #16221

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S96A, S100A, S107A, S111A, S115A, S119A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AMPK-γ1-HA

    Plasmid

    #40605

    Depositor

    Harvey Lodish

    Article

    Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

    Insert

    AMPK-γ1 (Prkag1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

21. Flag TSC2 wt

    Plasmid

    #12132

    Depositor

    Cheryl Walker

    Article

    Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

    Insert

    TSC2 (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFLAG-ZYG11B

    Plasmid

    #110550

    Purpose

    expresses a FLAG-tagged human ZYG11B protein in mamalian cells

    Depositor

    Edward Kipreos

    Article

    Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

    Insert

    ZYG11B (ZYG11B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. Marathon-RT for Split-PE (pEH59)

    Plasmid

    #190108

    Purpose

    Expresses Marathon-RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AMPK-β1-FLAG

    Plasmid

    #40602

    Depositor

    Harvey Lodish

    Article

    Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

    Insert

    AMPK-β1 (Prkab1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

25. Cdk4-DN-HA

    Plasmid

    #1877

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk4 (CDK4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative, D to N in conserved KLAD*FGLAR…

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cdk6-DN-HA

    Plasmid

    #1869

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative, D to N in conserved KLAD*FGLAR…

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

27. VAMP2-pHuji

    Plasmid

    #107369

    Purpose

    Synaptic vesicle protein VAMP2 fused to the red FP pH sensor pHuji

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    pHuji

    Expression

    Mammalian

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Myc tag S16-A PTB

    Plasmid

    #23025

    Depositor

    Douglas Black

    Article

    Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

    Insert

    PTB1 (PTBP1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed Serine 16 to Alanine

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

29. Flag Snail 2SA

    Plasmid

    #16219

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    2SA: S97A, S101A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

30. Flag Snail 4SA

    Plasmid

    #16220

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    4SA: S108A, S112A, S116A, S120A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

31. pa-G-GECO1.2

    Plasmid

    #67622

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    pa-G-GECO1.2
    

    Expression

    Mammalian

    Mutation

    V81H, M84K, L189F, T190S

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

32. FLAG Enthoprotin Full Length

    Plasmid

    #47419

    Depositor

    Peter McPherson

    Article

    Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

    Insert

    Enthoprotin
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

33. PCTAIRE-1-DN-HA

    Plasmid

    #1998

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    PCTAIRE-1 (CDK16 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative, D304N in conserved KLAD*FGLAR …

    Available Since

    Jan. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

34. 2XMyc-LRRK2-deltaHeat

    Plasmid

    #25068

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains the LRR-ROC-COR-Kinase-WD40 domains; Del…

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

35. Cdk6-DN

    Plasmid

    #1867

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Expression

    Mammalian

    Mutation

    Dominant negative, D to N in conserved KLAD*FGLAR…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

36. MSCV-ERK5-AEF

    Plasmid

    #31816

    Depositor

    Astar Winoto

    Article

    Sohn et al Mol Cell Biol. 2005 Oct;25(19):8553-66.

    Insert

    Erk5 (Mapk7 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    T219A and Y221F

    Promoter

    PCMV-LTR

    Available Since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

37. Flag Snail 4SE

    Plasmid

    #16223

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    4SE: S108E, S112E, S116E, S120E

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

38. 2XMyc-LRRK2-RCK-Y1699C

    Plasmid

    #25067

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the ROC-COR-Kinase domains aa 1329…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

39. PCTAIRE-1-DN

    Plasmid

    #1997

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    PCTAIRE-1 (CDK16 Human)
    

    Expression

    Mammalian

    Mutation

    Dominant negative, D304N in conserved KLAD*FGLAR …

    Available Since

    Jan. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

40. 2XMyc-LRRK2-COR-Y1699C

    Plasmid

    #25070

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the COR domain aa535-1838 Ty…

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only