We narrowed to 14,585 results for: pET

1. pETM-41P-DmGW182_700-861_N

   Plasmid

   #147016

   Purpose

   Bacterial Expression of DmGW182_700-861

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_700-861 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM-41P-DmGW182_744-840_N

   Plasmid

   #147017

   Purpose

   Bacterial Expression of DmGW182_744-840

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_744-840 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   One non silent mutation N799S and one silent muta…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETM-60-DmAgo1_572-984-optim_K

   Plasmid

   #146728

   Purpose

   Bacterial Expression of DmAgo1_572-984-optim

   Depositor

   Elisa Izaurralde

   Insert

   DmAgo1_572-984-optim (AGO1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM-60-NcQDE-2_506-646_J

   Plasmid

   #146646

   Purpose

   Bacterial Expression of NcQde-2_506-646

   Depositor

   Elisa Izaurralde

   Insert

   NcQde-2_506-646
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM-60-NusA-DmGW182_1-539_G

   Plasmid

   #146380

   Purpose

   Bacterial Expression of DmGW182_1-539

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-539 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM-60-P-HsRCK_296-472-mut_E

   Plasmid

   #146172

   Purpose

   Bacterial Expression of HsRck_296-472

   Depositor

   Elisa Izaurralde

   Insert

   HsRck_296-472 (DDX6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28c(plus)-DmAGO1-RC_F

   Plasmid

   #146262

   Purpose

   Bacterial Expression of DmAGO1-RC

   Depositor

   Elisa Izaurralde

   Insert

   DmAGO1-RC (AGO1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation R787C compared to sequenc…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28c(plus)-DmGW182_1-539_F

   Plasmid

   #146263

   Purpose

   Bacterial Expression of DmGW182_1-539

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-539 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETM-60-NusA-HsRad23-UBA_F

   Plasmid

   #146266

   Purpose

   Bacterial Expression of HsRad23-UBA

   Depositor

   Elisa Izaurralde

   Insert

   HsRad23-UBA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM-60-NusA-HsTNRC6A-UBA_F

    Plasmid

    #146267

    Purpose

    Bacterial Expression of HsTNRC6A-UBA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6A-UBA (TNRC6A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM-60-NusA-HsTNRC6B-UBA_F

    Plasmid

    #146268

    Purpose

    Bacterial Expression of HsTNRC6B-UBA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-UBA (TNRC6B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM-60-NusA-HsTNRC6C-UBA_F

    Plasmid

    #146269

    Purpose

    Bacterial Expression of HsTNRC6C-UBA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6C-UBA (TNRC6C Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM-11-6His-DmEDC3_331-680_D

    Plasmid

    #146100

    Purpose

    Bacterial Expression of DmEDC3_331-680

    Depositor

    Elisa Izaurralde

    Insert

    DmEDC3_331-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM-11-6His-DmTral_395-542_D

    Plasmid

    #146101

    Purpose

    Bacterial Expression of DmTral_395-542

    Depositor

    Elisa Izaurralde

    Insert

    DmTral_395-542 (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM-60-NusA-DmEDC3_331-680_D

    Plasmid

    #146102

    Purpose

    Bacterial Expression of DmEDC3_331-680

    Depositor

    Elisa Izaurralde

    Insert

    DmEDC3_331-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM-60-NusA-DmTral_395-542_D

    Plasmid

    #146103

    Purpose

    Bacterial Expression of DmTral_395-542

    Depositor

    Elisa Izaurralde

    Insert

    DmTral_395-542 (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28a(+)-His(6)-AtPCO1(wt)

    Plasmid

    #191798

    Purpose

    Full length wt AtPCO1 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO1
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a(+)-His(6)-AtPCO2(wt)

    Plasmid

    #191799

    Purpose

    Full length wt AtPCO2 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO2
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-28a(+)-His(6)-AtPCO3(wt)

    Plasmid

    #191800

    Purpose

    Full length wt AtPCO3 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO3
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 sf:em-blue-shift.2

    Plasmid

    #191919

    Purpose

    Encodes a GFP design, sf:em-blue-shift.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:em-blue-shift.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72A T108I Y145F V150I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

    Plasmid

    #178035

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted and Changed T(8…

    Promoter

    T7LacI

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-PLS-KP2-E67A_rpoA-U

    Plasmid

    #190959

    Purpose

    Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-PLS-KP6-A66S_rpoA-C

    Plasmid

    #190968

    Purpose

    Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-PLS-KP2-A66S_rpoA-U

    Plasmid

    #190957

    Purpose

    Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-PLS-KP3-A66S_rpoA-U

    Plasmid

    #190961

    Purpose

    Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-PLS-KP3-E67A_rpoA-U

    Plasmid

    #190963

    Purpose

    Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-PLS-KP6-E67A_rpoA-C

    Plasmid

    #190972

    Purpose

    Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Arabidopsis thaliana Atm3 pET21a+

    Plasmid

    #172321

    Purpose

    Expresses AtAtm3 in E.coli

    Depositor

    Douglas Rees

    Article

    Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

    Insert

    Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mTln1-F2F3-P229L-pET151

    Plasmid

    #177879

    Purpose

    Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mTln1-F2F3-P229S-pET151

    Plasmid

    #177878

    Purpose

    Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PET28a-cpLumiLuc-amylase sensor

    Plasmid

    #186293

    Purpose

    Expression alpha-amylase sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-amylase sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PET28a-cpLumiLuc-HSA sensor

    Plasmid

    #186294

    Purpose

    Expression Human serum albumin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-HSA sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET303-ColE1-TR-E192C-del1-40

    Plasmid

    #180235

    Purpose

    Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T and R domains (r…

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-15b-LanCL1-H219A

    Plasmid

    #154190

    Purpose

    To express LanCL1-H219A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His219->Ala219

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-15b-LanCL1-C322A

    Plasmid

    #154191

    Purpose

    To express LanCL1-C322A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys322->Ala322

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15 PemrR-gfp biosensor

    Plasmid

    #185793

    Purpose

    The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

    Depositor

    Steven Hallam

    Article

    Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

    Insert

    pEmrR-gfp
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PemrR

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15b MOSPD2 RD/LD [282-490]

    Plasmid

    #186470

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b MOSPD2 [282-490]

    Plasmid

    #186469

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET30-T7-lacO-His-sfGFP

    Plasmid

    #180754

    Purpose

    pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    G140C- Please see depositor comments

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge