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pENTR2b-EGFP

Plasmid

#215537

Purpose

Gateway entry vector with the EGFP fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-mTurquoise2

Plasmid

#215539

Purpose

Expression vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-YPet

Plasmid

#215540

Purpose

Gateway entry vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Hv_HPT:pGC1:Gus_introns

Plasmid

#215195

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

GA-stimulated regulatory protein

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

AtGC1

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pGC1:Gus_introns

Plasmid

#215181

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

GA-stimulated regulatory protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

AtGC1

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-8XHis-mCherry-Omp25

Plasmid

#236399

Purpose

Elutable MitoTag-AP system

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

8XHis-mCherry-Omp25

Use

Retroviral

Tags

8XHis-tag and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-tetR

Plasmid

#238043

Purpose

Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

ttrB

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUB_mCherry-Rluc100

Plasmid

#210600

Purpose

Expresses mCherry-Optimal Renilla

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

mCherry-Optimal Renilla

Use

Lentiviral and Luciferase

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc100_CSC

Plasmid

#210592

Purpose

Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)

Use

Tags

lambda HA

Expression

Insect

Mutation

Promoter

Ac5

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Use

Tags

Expression

Insect

Mutation

Promoter

Ac5

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_OptλHAramp-FLuc100

Plasmid

#210577

Purpose

Expresses Optimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal lambda HA-Optimal Firefly luciferase

Use

Tags

lambda HA

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_NonoptλHAramp-FLuc100

Plasmid

#210579

Purpose

Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Nonoptimal lambda HA-Optimal Firefly luciferase

Use

Lentiviral and Luciferase

Tags

lambda HA

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-pA

Plasmid

#210583

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-pA

Plasmid

#210584

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc0_CSC

Plasmid

#210589

Purpose

Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)

Use

Luciferase

Tags

lambda HA

Expression

Insect

Mutation

Promoter

Ac5

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Arg74ProLeuThr

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a MDHP_YEAST

Plasmid

#204233

Purpose

Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDHP_YEAST (MDH3 Budding Yeast)

Use

Tags

6X His

Expression

Bacterial

Mutation

The last 3 C-terminal residues (SKL) are the pero…

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine

Plasmid

#197681

Purpose

"Insert(high)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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