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We narrowed to 23,907 results for: Spr

Showing: 1661 - 1680 of 23907 results

  2. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

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  6. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101165-plasmid-map-sequence-id-199321

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  10. SiC-V1-Scr

    Plasmid
    #133042
    Purpose
    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133042-plasmid-map-sequence-id-264423

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  11. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  12. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    N/A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102563-plasmid-map-sequence-id-193893

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  15. pNHEJ-Cas12a

    Plasmid
    #158713
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158713-plasmid-map-sequence-id-320845

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  18. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  19. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Expression
    Mutation
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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Showing: 1661 - 1680 of 23907 results