We narrowed to 12,673 results for: sequence

1. pUC19 ACMV DNA-B 1.5mer

   Plasmid

   #159135

   Purpose

   Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

   Depositor

   Vincent Fondong

   Article

   Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

   Insert

   ACMV DNA-B 1.5mer
   

   Use

   Infectious clone for bombarding plants

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd

   Plasmid

   #182149

   Purpose

   To insert attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-A4b-attP-fwd pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

   Plasmid

   #182152

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-D1b-attB-rev pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

   Plasmid

   #182151

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-C2c-attBrev pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

   Plasmid

   #182150

   Purpose

   To install attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-B7b-attP-fwd pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. UASgal-A9-Pleum

   Plasmid

   #60343

   Purpose

   Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFX-GalT-Venus

   Plasmid

   #174472

   Purpose

   Express Venus with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with golgi apparatus-targeting sequence
   

   Use

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTCON2-BXG-2TAG

   Plasmid

   #158138

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first serine residue…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. KIAA-bio-His

    Plasmid

    #51808

    Purpose

    Expresses full-length Malectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    KIAA (MLEC Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-ecDHFR-G51PEKN

    Plasmid

    #118582

    Purpose

    Bacterial expression of E.coli DHFR G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G51 mutated to P51; Glu-Lys-Asn residues inserted…

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2 mir-1-1 reporter dme-mir-125

    Plasmid

    #46667

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-125
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCDH-bio-His

    Plasmid

    #53336

    Purpose

    Expresses full-length Protocadherin alpha-2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PCDH (PCDHA2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT1100

    Plasmid

    #8618

    Depositor

    Robert Hartley

    Article

    Hartley et al Protein Peptide Letters 1996 3:225-231

    Insert

    ribonuclease Sa3, barstar
    

    Use

    Tags

    phoA signal sequence to RNase Sa3

    Expression

    Bacterial

    Mutation

    In addition to the substitution of the barnase ge…

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

15. FLAG-ADA add back mutant pCMV5

    Plasmid

    #17554

    Depositor

    Domenico Accili

    Article

    Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

    Insert

    FLAG-FoxO1-ADA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    siRNA-resistant Foxo1 by replacing 3 residues (un…

    Promoter

    Available since

    Oct. 17, 2008

    Availability

    Academic Institutions and Nonprofits only

16. OTOA-bio-His

    Plasmid

    #53356

    Purpose

    Expresses full-length Otoancorin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    OTOA (OTOA Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2 mir-1-1 reporter mir30-wt

    Plasmid

    #46695

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir30-wt (MIR30A Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL80037

    Plasmid

    #68260

    Purpose

    Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Coding sequence, neomycin phophotransferase II (Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2 mir-1-1 reporter dme-mir-2a-1

    Plasmid

    #46664

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-2a-1
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EC.SurA.pTYB1.a21-281&390-428.L239R

    Plasmid

    #15959

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Escherichia coli surA delta PPIase domain 2
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    residues 282-389 deleted; L239R mutation

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only