62,255 results
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Plasmid#157792PurposeExpression of long isoform of BRD4DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
Plasmid#126706Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
opto-Rac1
Plasmid#135396PurposeExpresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.DepositorInsertopto-Rac1
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4(732)-8h
Plasmid#154103PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-G…PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
TB:5xNF- κβ_RE:MiniP
Plasmid#133878Purpose5 copies of the NF-κβ DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::NLuc
Plasmid#124533Purposep53 NLuc luciferase reporterDepositorInsertP53 NLuc reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA007
Plasmid#131380PurposeORFeus expression, WTDepositorInsertORFeus-Hs
TagsORF2-3xFlagExpressionMammalianAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-Fc(IgG1)
Plasmid#145164PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y530F
Plasmid#140318PurposeExpresses HA-tagged human Src-Y530F in mammalian cells for structure-function studiesDepositorAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only