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Plasmid#117778PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RA-HRas-IRES2-Lyn-iRFP682
Plasmid#180584PurposeRA-Hras and Lyn-iRFP682DepositorInsertRA-Hras and Lyn-iRFP682
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-iRFP670-GFP
Plasmid#111815PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
iRFP670
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-TagRFP-SpHis5
Plasmid#44854PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selectionDepositorInsertyoPA-TagRFP
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-emiRFP703-stop
Plasmid#186386PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mRFP-NLS-PCNA (2514)
Plasmid#61850Purposecell cycle markerDepositorInsertPCNA (PCNA Human)
UseTagsNLS and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
BSD-P2A-TagRFP-hSmad1-donor
Plasmid#203662PurposeDonor plasmid for CRISPR knock-inDepositorInsertsmad1 (SMAD1 Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS1026-Tier1-PhCMV-3xNLS-iRFP670
Plasmid#169532PurposeTier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).DepositorInsertPCMV-driven nucleus-localized far-red fluorescent protein iRFP670
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-mRFP-gsk3 beta S9A
Plasmid#24521DepositorInsertGSK3beta(S9A) (GSK3B Human)
UseAdenoviralTagsmRFPExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-smURFP-P2A-GFP
Plasmid#197151PurposeExpresses the protein of smURFP-P2A-GFP in mammalian cellsDepositorInsertsmURFP-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-NLS-tdiRFP670-2A-LBR
Plasmid#82620PurposeExpresses NLS-tdiRFP-2A-LBR in mammalian cellsDepositorInsertNLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKEW321-MbCas12a-RFP-GFP-array
Plasmid#115661PurposeEncodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1DepositorInsertGFP and RFP array for MbCas12
UseCRISPRTagsExpressionBacterialMutationPromoterPlacAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-RFPnls-PQRv2-mCD8:GFP
Plasmid#73974PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.DepositorInsertssfGFP
RFP
UseTagsmCD8 and nlsExpressionInsectMutationPromoter20XUAS-HSAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP/RFP-miR-E1-1int
Plasmid#19819DepositorInsertmiRNA
UseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-RFP-miR-223
Plasmid#97148PurposemiR-223 expressionDepositorInsertmiR-223
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral nontargeting shRNA RFP i670
Plasmid#155287PurposeLentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-Met-Eif4a1-tdiRFP670-PEST
Plasmid#122371PurposeTargeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.DepositorInsert2A-tdiRFP-PEST-2A with homology arms for Eif4a1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only