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pJM105: pTac RFP on pCM66T
Plasmid#74746PurposepCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpTacAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-501V2-FLAG
Plasmid#177096Purpose501V2 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein 501V2 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQC V5 MCS IRES Puro
Plasmid#110342PurposeĪ³-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST12.2-DYRK2 (469T)
Plasmid#174850PurposeExpression of DYRK2DepositorInsertDYRK2 (DYRK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMT-HA-STX4
Plasmid#89311PurposeExpresses HA tagged syntaxin 4 in Drosophila cellsDepositorInsertSTX4 (Syx4 Fly)
UseTagsHA tagExpressionInsectMutationPromoterP-MT (Metallothionein)Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-B.1.617.1-FLAG
Plasmid#177098PurposeB.1.617.1 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein B.1.617.1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAAV-EF1-fDIO-mNeon-WPRE-PolyA
Plasmid#121546PurposeSuper-bright fluorescence protein for tracking and visualizationDepositorInsertcre-dependent mNeon
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX383 Lactobacillus johnsonii Cas9
Plasmid#68320PurposeLactobacillus johnsonii Cas9DepositorInsertLjCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir
Plasmid#178044PurposeCAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertPten_1523 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
4MX2
Plasmid#60102PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLdBPK_040440.1:MAC04A-B07:C226244
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV Tor1a-Myc
Plasmid#175134PurposeLentiviral expression of Myc-tagged mouse Tor1aDepositorInsertTor1a (Tor1a Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-F979A/N980A shRescue
Plasmid#28143DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationA489V, F979A and N980A; aa 308 and 309 mutated foā¦PromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pBCSK_HIS3_II
Plasmid#109059Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxLOX1
Plasmid#104975PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1745 (MXAN_1745 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxLOX2
Plasmid#104976PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1744 (MXAN_1744 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P85(W583del)
Plasmid#40229DepositorInsertP85(W583del) (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationdeleted amino acid 583PromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa-C1V1::FusionRed::Kv2.1
Plasmid#102771Purpose"Activate neurons with 2p stimulation"DepositorInsertC1V1::FusionRed::Kv2.1
UseAAVTagsFusionRed and Kv2.1 traficking domainExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.3_HES2
Plasmid#124070PurposeHES2 with normal stop codonDepositorInsertHES2 (HES2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRKVSV-PTEN
Plasmid#50538DepositorInsertPTEN (PTEN Human)
UseTags2X VSVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV hH1-ARRET
Plasmid#111374PurposeARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertARRET w/o polyA
UseAdenoviralTagsExpressionMutationPromoterhH1Available sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/mKO2
Plasmid#110353PurposeDoxycycline inducible mammalian retroviral expression of mKO2 with FLAG tagDepositorInsertmonomeric Kusabira Orange 2
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Plekhm1 - N-terminal pGEX 4T2-GST
Plasmid#73830PurposeExpress human Plekhm1 N-part in bacterial cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGstExpressionBacterialMutationto vector was cloned N-terminal part of human Pleā¦PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer
Plasmid#58837PurposeExpresses GFP-Tagged Human PLC delta PH domain in yeastDepositorInsert2x Phospolipase C delta1 PH domain (Plcd1 Rat)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX382 Staphylococcus pseudintermedius Cas9
Plasmid#68319PurposeStaphylococcus pseudintermedius Cas9DepositorInsertSpsCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV cloning vector with BioBrick polycistronic restriction sites (9O)
Plasmid#48289DepositorTypeEmpty backboneUseTagsHis6 and MocrExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
PX386 Lactobacillus farciminis Cas9
Plasmid#68323PurposeLactobacillus farciminis Cas9DepositorInsertLfCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX395 Roseburia intestinalis Cas9
Plasmid#68332PurposeRoseburia intestinalis Cas9DepositorInsertRiCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX393 Gluconacetobacter diazotrophicus Cas9
Plasmid#68330PurposeGluconacetobacter diazotrophicus Cas9DepositorInsertGdCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only
