We narrowed to 3,519 results for: Braf;

1. p3E-DysF

   Plasmid

   #109544

   Purpose

   Multisite gateway vector for 3' tagging with Dysferlin

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Dysferlin (dysf Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3E-Clta

   Plasmid

   #109585

   Purpose

   Multisite gateway vector for 3' tagging with Clathrin light chain a

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Clathrin light chain a (clta Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pdU6-2_sgRNA-d5-HT1AR

   Plasmid

   #221820

   Purpose

   Plasmid to express gRNA (gaccagtccactaccgcagc) for editing at the end of Drosophila 5-HT1AR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT1AR gRNA (5-HT1A Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   zebrafish U6 promoter (U6b)

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdU6-2_sgRNA1-d5-HT1BR

   Plasmid

   #221821

   Purpose

   Plasmid to express gRNA1 (gaaaatttgatttcaactga) for editing at the end of Drosophila 5-HT1BR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT1BR gRNA1 (5-HT1B Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   zebrafish U6 promoter (U6b)

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pdU6-2_sgRNA2-5-HT2AR-isoformD

   Plasmid

   #221824

   Purpose

   Plasmid to express gRNA2 (ctgaagacataattacgtgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT2AR isoform D gRNA2 (5-HT2 Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   zebrafish U6 promoter (U6b)

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pdU6-2_sgRNA1-5-HT2AR-isoformBFH

   Plasmid

   #221825

   Purpose

   Plasmid to express gRNA1 (cgctatcggtctgtgacaga) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT2AR isoforms BFH gRNA1 (5-HT2 Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   zebrafish U6 promoter (U6b)

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pdU6-2_sgRNA-d5-HT7R

   Plasmid

   #221829

   Purpose

   Plasmid to express gRNA (ggcgagggagagctttctct) for editing at the end of Drosophila 5-HT1AR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT7R gRNA (5-HT7 Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   zebrafish U6 promoter (U6b)

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FLAG3-drAgo2DY-EF

   Plasmid

   #221733

   Purpose

   Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2DY-EF (ago2 Zebrafish)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D651E and Y680F in active site

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2+-FLAG3-hsAgo2F-Y

   Plasmid

   #221722

   Purpose

   Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2F-Y (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   F666Y in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+-FLAG3-hsAgo2E-D

    Plasmid

    #221721

    Purpose

    Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2E-D (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    E637D in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+-FLAG3-hsAgo2D-A

    Plasmid

    #221720

    Purpose

    Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2D-A (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D669A in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+-FLAG3-hsAgo2

    Plasmid

    #221719

    Purpose

    Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2 (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5E-dr_Unc (JDW 1365)

    Plasmid

    #224482

    Purpose

    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Unc-503 promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196112

    Purpose

    mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zmiRFP-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196111

    Purpose

    mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zmiRFP2-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196109

    Purpose

    mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zTagRFP658-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196110

    Purpose

    mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zemiRFP2-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mKate2-DrCavin1b in pT3TS-Dest

    Plasmid

    #194295

    Purpose

    In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1b (cavin1b Zebrafish)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-mCherry-Cenexin-S796A

    Plasmid

    #186434

    Purpose

    Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 796 to Alanine

    Promoter

    CMV IE94

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-ARF1-DN

    Plasmid

    #109590

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative ARF1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF1 (arf1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p3E-ARF6-DN

    Plasmid

    #109592

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p3E-SERCA1

    Plasmid

    #109552

    Purpose

    Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Tol2-BetaActin-SecA5-YFP

    Plasmid

    #105664

    Purpose

    Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

    Depositor

    Qing Deng

    Article

    Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

    Insert

    Secreted Annexin5-EYFP (ANXA5 Human)
    

    Use

    Tol2-gateway

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Beta-Actin

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2+ Zf ctnna1L344P

    Plasmid

    #185407

    Purpose

    Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    ctnna1 (ctnna1 Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 344 to Proline

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid

    #85818

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc oDam_f_GFPoNLS
    

    Use

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato

    Plasmid

    #105644

    Purpose

    Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    CMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    CMV:ikk1FL(C178A)-tdTomato

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

27. EGFP-Bin1b

    Plasmid

    #176014

    Purpose

    Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. zgc:153086_R (OZ512)

    Plasmid

    #27187

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zgc:153086
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

29. zgc:153086_L (OZ511)

    Plasmid

    #27186

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zgc:153086
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

30. SDC3_L (OZ595)

    Plasmid

    #35235

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting SDC3
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

31. SDC3_R (OZ596)

    Plasmid

    #35236

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting SDC3
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

32. Tg(Sox17::EMTB-3xGFP)

    Plasmid

    #221190

    Purpose

    To specifically label microtubules (MTs) in Kupffer's Vesicle (KV)

    Depositor

    Heidi Hehnly

    Article

    Wu et al Development. 2025 May 15;152(10):dev2046

    Insert

    Ensconsin Microtubule-Binding Protein (MAP7 Human)
    

    Use

    Zebrafish

    Tags

    3 EGFP

    Expression

    Mutation

    Microtubule binding-domain of ensconsin (A.A. 18…

    Promoter

    Sox17

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS2+-FLAG3-hsAgo2EF-DY

    Plasmid

    #221723

    Purpose

    Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2EF-DY (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    E637D and F666Y in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mKate2-DrCav1 in pT3TS-Dest

    Plasmid

    #194293

    Purpose

    In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin (cav1.S Frog)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p3E-DrCavin1a

    Plasmid

    #194291

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1a (cavin1a Zebrafish)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2z-lmo2

    Plasmid

    #164657

    Purpose

    for the synthesis of zebrafish lmo2 mRNA

    Depositor

    Didier Stainier

    Article

    Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

    Insert

    lmo2 (lmo2 Zebrafish)
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SITS-EGFP-Bin1a

    Plasmid

    #176020

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SITS-mCherry-Bin1a

    Plasmid

    #176024

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG109 H2B-FAST

    Plasmid

    #130722

    Purpose

    Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    H2B-FAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SITS-mCherry-Bin1b

    Plasmid

    #176025

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge