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Plasmid#167136PurposeA tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ1
Plasmid#167130PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-134/150TAG
Plasmid#174077PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationD134TAG + N150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC5
Plasmid#118863Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
Plasmid#74010PurposeTissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoterDepositorInserts5xUAS
Cas9
Cre
U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Puro
Plasmid#167863PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
FL-BCAN-lentiviral-GFP
Plasmid#29704DepositorInsertBrevican (BCAN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVH1002-Tier2(pUC ori)
Plasmid#169600PurposeTier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVE-eGFP
Plasmid#52581PurposeLentiviral transfer vector driving expression of eGFP in mammalian cellsDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBJ6
Plasmid#167135PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-LASV-GPC
Plasmid#215690PurposeProduces the Lassa virus glycoproteinDepositorInsertLASV GP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-IRES-Gβγ-BERKY3
Plasmid#158426PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCl-MCS_miniCMV_TagBFP
Plasmid#134986PurposeThe plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vectorDepositorInsertTagBFP
UseLentiviralTagsExpressionMutationPromoterminCMVAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH231-Tier1-PmPGK1-mTagBFP2
Plasmid#169520PurposeTier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).DepositorInsertPPGK-driven monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb RxCas13d + RxCas13d guide RNA
Plasmid#176303PurposeExpresses RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
UseTagsHA tagExpressionInsectMutationPromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK451 (BSD-P2A-dTAG)
Plasmid#214381PurposedTAG for N-terminal taggingDepositorInsertBSD-P2A-dTAG
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
IZ501: pMVP/Lenti-DEST
Plasmid#121848PurposepMVP destination vector, empty Lentivirus vector backboneDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorInsertSaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only