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Showing: 2551 - 2580 of 20871 results

  8. pGEX-2T-TRX-C751-E3616S

    Plasmid
    #182844
    Purpose
    mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    TRX (w Fly)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182844-plasmid-map-sequence-id-360053

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  12. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

    Plasmid
    #108374
    Purpose
    Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    108374-plasmid-map-sequence-id-214321

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  13. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59894-plasmid-map-sequence-id-108252

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  14. pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)

    Plasmid
    #52129
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C667…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)

    Plasmid
    #52130
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C643…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)

    Plasmid
    #52131
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C631…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)

    Plasmid
    #52133
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and C478S, C631S, C643S,…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

    Plasmid
    #52134
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-281 and C605S, C631S, C643S …
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid
    #82031
    Purpose
    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Non phosphorylatable mutations on 3 of the main A…
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82031-plasmid-map-sequence-id-155380

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  27. pGal-Trans-Myc-HA-GFP11-KDEL

    Plasmid
    #177724
    Purpose
    ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    Use
    Tags
    GFP11, HA, KDEL, and Myc
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177724-plasmid-map-sequence-id-352616

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  28. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid
    #89890
    Purpose
    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    HA-BirA-2A-citrine
    Use
    Unspecified
    Tags
    Citrine
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89890-plasmid-map-sequence-id-181309

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Showing: 2551 - 2580 of 20871 results