We narrowed to 13,711 results for: cas9

1. pAAV-SMVP-Cas9N-U6-gRNA M3

   Plasmid

   #80936

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Icam 2 SaCas9 YAP sgRNA2

   Plasmid

   #99735

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

   Plasmid

   #222993

   Purpose

   Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

   Plasmid

   #222991

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

   Plasmid

   #141048

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF1-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HiFi-nCas9-NG_P2A-mCherry

   Plasmid

   #219936

   Purpose

   Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   HiFi-nCas9(D10A/R691A)

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

   Plasmid

   #71828

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScRad52-SpCas9 (pXK-1646)

    Plasmid

    #208823

    Purpose

    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

    Depositor

    Kun Xu

    Article

    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

    Type

    Empty backbone

    Use

    CRISPR; Cas9

    Tags

    Rad52

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. A3Ai-Cas9n-UGI-NLS

    Plasmid

    #109425

    Purpose

    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI Cas9 H2B-RFP

    Plasmid

    #92397

    Purpose

    CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDL17_Cas9-His Δcys M1C

    Plasmid

    #206251

    Purpose

    Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, C574S

    Promoter

    T7

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-Cas9C-VPR

    Plasmid

    #80933

    Purpose

    Expresses Cas9C-VPR in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C-VPR
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid

    #89637

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

    Plasmid

    #222992

    Purpose

    Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CAG-SpCas9-BlastR

    Plasmid

    #107190

    Purpose

    Confers constitutive expression of SpCas9

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    SpCas9 (NEWENTRY )
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Chicken ß-actin

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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