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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Publication

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Publication

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Publication

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Promoter

CMV

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAG-FAST-p10

Plasmid

#89152

Purpose

Express p10-FAST in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NBV p10-FAST

Expression

Mammalian

Promoter

CAG promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAC-BETAipi

Plasmid

#53277

Purpose

Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

idi

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Publication

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IFT88 (Ift88 Mouse)

Tags

TagRFP.T

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Publication

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

Plasmid

#55212

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, High pI protein 69, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

Plasmid

#48288

Depositor

Scott Gradia

Type

Empty backbone

Tags

FLAG

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Publication

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Ca-FLARE (TF)

Plasmid

#92213

Purpose

Transcription factor component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223-K-RAS V12

Plasmid

#31200

Purpose

Gateway donor vector containing KRAS G12V.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway Cloning

Mutation

G12V constitutively active

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse ULK1 (Ulk1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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