We narrowed to 5,538 results for: pcas

1. pCASP-HilA empty vector

   Plasmid

   #153333

   Purpose

   Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Type

   Empty backbone

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCASP-SptP167-3G124-HilA

   Plasmid

   #153322

   Purpose

   Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP167-3G124 DARPin
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasper-pNos-PCP-eGFP

   Plasmid

   #180274

   Purpose

   to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

   Depositor

   Hernan Garcia

   Article

   Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

   Insert

   PCP-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS24: pCascade(PmcCAST)_entry

   Plasmid

   #168157

   Purpose

   Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScalps eSpCas9_Zsgreen gRNA scaffold

   Plasmid

   #188449

   Purpose

   Expression of eSpCas9 tagged with Zsgreen

   Depositor

   Ajit Chande

   Article

   Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1-alpha

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

   Plasmid

   #148855

   Purpose

   Mammalian Expression of HsAGO3-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO3-sgRNA (AGO3 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASP-SptP120-3m160-HilA

   Plasmid

   #153325

   Purpose

   Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-3m160 DARPin
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Trex2-bpA-EF1BFP

    Plasmid

    #111144

    Purpose

    Expression of mouse Trex2 and BFP reporter in mammalian cells

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Inserts

    mouse Trex2 coding region (Trex2 Mouse)
    BFP (Rnf112 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid

    #128174

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-SRGAP2A-FBAR-mRFP

    Plasmid

    #168502

    Purpose

    Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A-FBAR (SRGAP2 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Osa-pCAMBIA-1301-STTM159

    Plasmid

    #84195

    Purpose

    Disturb the expression of miR159 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR159
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pCAGGS-PB1-MontiRed-LOV2

    Plasmid

    #178872

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

    Plasmid

    #128171

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCa4B2G-QUAS-DSCP-FLPo

    Plasmid

    #24359

    Depositor

    Liqun Luo

    Article

    Potter et al Volume 141, Issue 3, 536-548, 30 April 2

    Insert

    QUAS-DSCP-FLPo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Osa-pCAMBIA-1301-STTM394

    Plasmid

    #84204

    Purpose

    Disturb the expression of miR394 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR394
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid

    #121961

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    R911A

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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