We narrowed to 1,843 results for: rigi

1. pET SUMO-Doc2β C2AB (G361C)

   Plasmid

   #213631

   Purpose

   Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   Doc2β C2AB (G361C) (Doc2b Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   G361C, all original cysteines mutated to alanines

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUGP1.1

   Plasmid

   #196615

   Purpose

   Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CLC-EGFP206(5)

   Plasmid

   #193573

   Purpose

   Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 207-211

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CLC-EGFP206(6)

   Plasmid

   #193574

   Purpose

   Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 207-211

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CLC-EGFP206(7)

   Plasmid

   #193575

   Purpose

   Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 207-211

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. vPyACR_2164382

   Plasmid

   #153029

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_2164382
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_2164382 was C-terminally truncated to incl…

   Promoter

   CMV (+ enhancer)

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. vPyACR_21821

   Plasmid

   #153030

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_21821
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_21821 was C-terminally truncated to includ…

   Promoter

   CMV (+ enhancer)

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC-G418-YR

   Plasmid

   #61767

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   nptII gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV2-CAG-3xNLS-vsfGFP-0

   Plasmid

   #191097

   Purpose

   To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-vsfGFP-0
   

   Use

   AAV

   Tags

   Green fluorescent protein bound to enhancer nanob…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV2-CAG-3xNLS-AausFP1

    Plasmid

    #191096

    Purpose

    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-AausFP1
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALS2-sfGFP 150TAG

    Plasmid

    #197574

    Purpose

    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP1-bio

    Plasmid

    #47709

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH733-2µ-RLuc/maxCFLuc

    Plasmid

    #40608

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC-SUR-YR

    Plasmid

    #61768

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Sur gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    Magnaporthe oryzae ILV promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD2_miniluc_newP7

    Plasmid

    #174105

    Purpose

    adding the mini luciferase and mini promoter to the MPRA lenti construct

    Depositor

    Paul Khavari

    Article

    Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

    Insert

    mini luciferase mini-promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PF3D7_1136200-bio

    Plasmid

    #47730

    Purpose

    Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PF3D7_1136200
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin replaced…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSICO-CPSF6-358-iRFP670

    Plasmid

    #110694

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Truncation of CPSF6 isoform at amino acid 358

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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