We narrowed to 24,629 results for: Nes

1. pJMC-L2-Donor-BsaI

   Plasmid

   #197840

   Purpose

   Donor vector to restore L2 destination via BsaI

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   None
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-4T-1-3xVSVG-GSK3(1-97)

   Plasmid

   #129582

   Purpose

   Encodes VSVG tagged GSK3(1-97) for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   GSK3a(1-97) (GSK3A Human)
   

   Use

   Tags

   GST and VSVG

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-29b-3xVSVG-GSK3(1-97)

   Plasmid

   #129583

   Purpose

   Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   GSK3a(1-97) (GSK3A Human)
   

   Use

   Tags

   6xHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMaCTag-21

   Plasmid

   #120000

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with 24xSunTag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   24xSunTag
   

   Use

   Pcr template

   Tags

   24xSunTag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-32

   Plasmid

   #120011

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with S-Tag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   S-Tag
   

   Use

   Pcr template

   Tags

   S-Tag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMaCTag-31

   Plasmid

   #120010

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with V5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   V5
   

   Use

   Pcr template

   Tags

   V5

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMaCTag-30

   Plasmid

   #120009

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with VSV-G

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   VSV-G
   

   Use

   Pcr template

   Tags

   VSV-G

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMaCTag-20

   Plasmid

   #119999

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMaCTag-16

   Plasmid

   #119995

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with Dronpa3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Dronpa3
   

   Use

   Pcr template

   Tags

   Dronpa3

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-03

    Plasmid

    #119982

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxCerulean3
    

    Use

    Pcr template

    Tags

    moxCerulean3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMaCTag-02

    Plasmid

    #119981

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with TagBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TagBFP
    

    Use

    Pcr template

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ReNL-Actin_pcDNA3

    Plasmid

    #89531

    Purpose

    Red color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tubulin-OeNL_pcDNA3

    Plasmid

    #89523

    Purpose

    Orange color variant of bright luminescent protein, fused to b-tubulin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    b-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEN_TTmiRc2 3XFLAG-EGFP-NLS

    Plasmid

    #87778

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    eGFP
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

    Plasmid

    #87777

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7-EE
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB1_omega2R

    Plasmid

    #68237

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFLIP34

    Plasmid

    #65501

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFLIP35

    Plasmid

    #65502

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-TFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFLIP36

    Plasmid

    #65504

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-Cer-cMyc

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLIP42

    Plasmid

    #65617

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-mCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTH490-tQSRTL

    Plasmid

    #29699

    Depositor

    Tobias von der Haar

    Article

    Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

    Insert

    tR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p-rlf-1

    Plasmid

    #14056

    Depositor

    Richard Hynes

    Article

    Schwarzbauer et al Cell. 1983 Dec . 35(2 Pt 1):421-31.

    Insert

    Rat Fibronectin (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

23. pcFNE3B (chick fibronectin E3B)

    Plasmid

    #14064

    Depositor

    Richard Hynes

    Article

    Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

    Insert

    Chicken Fibronectin EIIIB (FN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pJK384.4

    Plasmid

    #155369

    Purpose

    dCas9 + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR221-Renilla

    Plasmid

    #79496

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Renilla Luciferase
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 6xHis-SNAP-PI(4,5)P2 Probe

    Plasmid

    #211513

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    PLCdelta1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 6xHis-SNAP-PI Probe

    Plasmid

    #211507

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BcPI-PLC-ANH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 6xHis-SNAP-PI(4)P Probe

    Plasmid

    #211509

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SidC (P4C)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. JD136

    Plasmid

    #229497

    Purpose

    Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, L4-22k/33k, VA RNA I
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMaCTag-P11

    Plasmid

    #120022

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LOTUS-V/pcDNA3

    Plasmid

    #87127

    Purpose

    Bioluminescent and ratiometric voltage indicator

    Depositor

    Takeharu Nagai

    Article

    Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

    Insert

    LOTUS-V
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMaCTag-P19

    Plasmid

    #124789

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK02

    Plasmid

    #105133

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMaCTag-P07

    Plasmid

    #124788

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge