We narrowed to 51,827 results for: ANG

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pcDNA3-RIα-ICUE4

Plasmid

#181848

Purpose

ICUE4 cAMP sensor tethered to the PKA RIα subunit.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-ICUE4 (RAPGEF3, PRKAR1A Human)

Tags

ECFP, PKA RIα, and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-KMS

Plasmid

#188038

Purpose

Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124428

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-cj-E sgRNA

Plasmid

#169915

Purpose

Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124434

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Expression

Insect

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

Plasmid

#112211

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

C-terminus of dCas9

Use

AAV

Mutation

D10A

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

Plasmid

#124429

Purpose

Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(TEV cleavage seq) T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124431

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Zebrafish (if expression level is too high in zeb…

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Expression

Worm

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Expression

Worm

Promoter

myo-2

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-AVI

Plasmid

#160477

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-SD1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PDE4D2cat-ICUE4

Plasmid

#181847

Purpose

ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)

Tags

ECFP, PDE4D2(86-418), and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.1

Plasmid

#172319

Purpose

expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.1 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

Promoter

CMV

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Mu

Plasmid

#177578

Purpose

expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Mu variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Lambda

Plasmid

#177579

Purpose

expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Lambda variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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