We narrowed to 4,666 results for: LUM;

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FEMP

Plasmid

#191973

Purpose

FRET-based indicator of ER-mitochondria proximity (FEMP)

Depositor

Luca Scorrano

Article

Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

AkapYFP-Tav2a-CFP-sac1

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL

Plasmid

#169105

Purpose

Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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RA-Sec61β

Plasmid

#153978

Purpose

Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

Sec61 Transolocon Subunit Beta (SEC61B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2-GFP

Plasmid

#204202

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

multiple silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGF-mSox2-ONL

Plasmid

#65719

Purpose

mSox2 reporter plasmid (expresses ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Sox2 promoter and ONL

Use

Lentiviral and Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL3

Plasmid

#109024

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen R119A

Plasmid

#226572

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

R119A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen D188A

Plasmid

#226573

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

D188A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen F191A

Plasmid

#226571

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

F191A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PERK 2: PERK.K618A.9E10.pCDNA

Plasmid

#21815

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (K618A) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

kinase dead K618A mutant

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 WT

Plasmid

#186467

Purpose

Expression of human MOSPD2 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmGFP-Sec16S

Plasmid

#15775

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16S (SEC16B Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-UTR

Plasmid

#121142

Purpose

Mammalian expression of GFP tagged CD274 with its own 3'UTR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

CD274 with its own 3'UTR (CD274 Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GB-Dcp1b

Plasmid

#153979

Purpose

Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1b (DCP1B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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