We narrowed to 14,123 results for: cas9

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MiniCoopR 2xU6:gRNA, mitfa:Cas9

Plasmid

#118844

Purpose

Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a7

Plasmid

#124846

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-10xGCN4_Hygro

Plasmid

#192651

Purpose

3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-10xGCN4

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_CMV-Cas9-P2A-HygR

Plasmid

#164133

Purpose

Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSaCas9-1xPP7-2x3'UTR

Plasmid

#122947

Purpose

For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-PP7-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-D3A-3L-dCas9

Plasmid

#240530

Purpose

Expresses MMLVgag–DNMT3A-3L-dCas9 for producing RENDER-DNMT3A-3L-dCas9

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-D3A-3L-dCas9

Tags

FLAG, HA

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(WT)-EGFP

Plasmid

#89633

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-U6-sgHTT1-7sk-sgCas9

Plasmid

#190900

Purpose

AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA (HTT Human, spCas9)

Use

AAV and CRISPR

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS2699-CMV-Nme2Cas9-E932R

Plasmid

#228720

Purpose

Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2696-CMV-Nme2SmuCas9-D56R

Plasmid

#228717

Purpose

Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2698-CMV-Nme2Cas9-D56R

Plasmid

#228719

Purpose

Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-P2A-puro

Plasmid

#214358

Purpose

Expresses SpCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9 and puromycin resistance genes

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato SOX2-sgRNA

Plasmid

#196190

Purpose

Editing human SOX2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2 (SOX2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p-dCas9-HDAC1-H141A-Hygro

Plasmid

#104417

Purpose

transient expression of dCas9-dHDAC1 fusion protein

Depositor

Stephan Beck

Insert

dHDAC1 (HDAC1 Human)

Use

CRISPR

Expression

Mammalian

Mutation

H141A

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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PX393 Gluconacetobacter diazotrophicus Cas9

Plasmid

#68330

Purpose

Gluconacetobacter diazotrophicus Cas9

Depositor

Feng Zhang

Insert

GdCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAC152-dual-dCas9VP64-sgExpression

Plasmid

#48238

Purpose

Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA Tag, HA-Tag, and VP64

Expression

Mammalian

Mutation

D10A;H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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PX383 Lactobacillus johnsonii Cas9

Plasmid

#68320

Purpose

Lactobacillus johnsonii Cas9

Depositor

Feng Zhang

Insert

LjCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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