We narrowed to 5,820 results for: pcas

1. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SARS2-S-FKO (NC-flag)

   Plasmid

   #159363

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Tags

   Flag and Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-SARS2-S-G614 (NC-flag)

   Plasmid

   #156419

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 35S::Cel1SP-GeNL/pCAMBIA1301

   Plasmid

   #190069

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Cel1 signal peptide-Green-enhanced Nano-lantern
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Flag-hsDicer (E1564A)

   Plasmid

   #41587

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E1564A

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Mutation

   D156R, R883K, R887A

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASP-SptP120-3G124-HilA

   Plasmid

   #153323

   Purpose

   Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-3G124 DARPin
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT344_pattB-pCA-GFP-pA-FRT5

   Plasmid

   #52550

   Purpose

   for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Expression

   Mammalian

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCaSpeR4 mito roGFP2-Grx1

   Plasmid

   #65000

   Purpose

   Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-PCAF (delta5-53a.a.)

    Plasmid

    #65388

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    PCAF (KAT2B Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    lost 13-159bp of the ORF

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-Flag-hsDicer (D1320A)

    Plasmid

    #41586

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1320A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-attBr-Cre-rc-attP

    Plasmid

    #51264

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-Cre-rc-attP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaSper4 cyto roGFP2-Orp1

    Plasmid

    #64998

    Purpose

    Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAMBIA-FLS2prom::FLS2-3xmyc

    Plasmid

    #86158

    Purpose

    FLS2 expression

    Depositor

    Silke Robatzek

    Article

    Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Tags

    3xmyc

    Expression

    Plant

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMMACE DEST CMV SpCas9

    Plasmid

    #206268

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9
    

    Use

    CRISPR; Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCA528-AMOT(1-300)-sortase

    Plasmid

    #166706

    Purpose

    expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT aa 1-300
    

    Tags

    His-SUMO and Sortase tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-FLAG-BMAL1-S513A

    Plasmid

    #186831

    Purpose

    Expresses FLAG-BMAL1-S513A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-FLAG-BMAL1-S515A

    Plasmid

    #186832

    Purpose

    Expresses FLAG-BMAL1-S515A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 515 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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