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We narrowed to 5,049 results for: golden gate

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Showing: 4401 - 4450 of 5049 results

  2. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid
    #182356
    Purpose
    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Expression
    Worm
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182356-plasmid-map-sequence-id-361657

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  3. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

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  4. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

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  6. pYSD328

    Plasmid
    #253040
    Purpose
    Encodes the Aga2 signal peptide, anti-ALFA-tag nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-ALFA-tag nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253040-plasmid-map-sequence-id-508937

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  7. pYSD894

    Plasmid
    #253020
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga2 display anchor in a Met15, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253020-plasmid-map-sequence-id-508728

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  8. pYSD892

    Plasmid
    #253018
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga1 del N181aa anchor in a HIS3, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253018-plasmid-map-sequence-id-508727

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  9. pYSD891

    Plasmid
    #253017
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-649 stalk display anchor in a LEU2, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253017-plasmid-map-sequence-id-508726

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  10. pYSD728

    Plasmid
    #253050
    Purpose
    Encodes OST1 Sig Pep, LC Cutinase-WT, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Leaf compost cutinase
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253050-plasmid-map-sequence-id-508705

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  11. pYSD326

    Plasmid
    #253039
    Purpose
    Encodes the OST1-pre / MFalpha1 Pro signal peptide, Protein A ZZ domain, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Protein A 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253039-plasmid-map-sequence-id-507381

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  12. pYSD329

    Plasmid
    #253041
    Purpose
    Encodes the Aga2 signal peptide, anti-mCherry_LaM4 nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-mCherry_LaM-4 nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253041-plasmid-map-sequence-id-507382

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  13. pYSD332

    Plasmid
    #253042
    Purpose
    Encodes HA-tagged Aga2 yeast surface display anchor protein, Anti-GFP nanobody as a C-terminal fusion, in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2 Anti-GFP nanobody
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253042-plasmid-map-sequence-id-507383

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  14. pYSD091

    Plasmid
    #252981
    Purpose
    Encodes S. cerevisiae HA-tagged Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    HA-tagged Aga1 del N181aa display anchor
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252981-plasmid-map-sequence-id-507379

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  15. pYSD092

    Plasmid
    #252982
    Purpose
    Encodes S. cerevisiae 2xFLAG-tagged Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    2xFLAG-tagged Aga1 del N181aa display anchor
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252982-plasmid-map-sequence-id-507380

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  16. pYSD893

    Plasmid
    #253019
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-SED1 display anchor in a URA3, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253019-plasmid-map-sequence-id-507368

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  17. pYSD373

    Plasmid
    #253048
    Purpose
    Encodes an AGA2 Signal Peptide, Anti-mCherry_nanobody_LaM-4 and 2xFLAG-tagged-649-stalk anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-mCherry_LaM-4 nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253048-plasmid-map-sequence-id-507362

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  18. pYSD353

    Plasmid
    #253045
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, FLAG-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-GFP nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253045-plasmid-map-sequence-id-507359

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  19. pYSD354

    Plasmid
    #253046
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, MYC-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-GFP nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253046-plasmid-map-sequence-id-507360

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  20. pYSD371

    Plasmid
    #253047
    Purpose
    Encodes an AGA2 Signal Peptide, Anti- ALFAtag_nanobody and 2xFLAG-tagged-649-stalk anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-ALFA-tag nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253047-plasmid-map-sequence-id-507361

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  21. pYSD093

    Plasmid
    #252983
    Purpose
    Encodes MYC-tagged S. cerevisiae Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MYC-tagged S. cerevisiae Aga1 del N181aa display anchor
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252983-plasmid-map-sequence-id-507347

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  22. pYSD315

    Plasmid
    #253034
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    mRuby2-649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253034-plasmid-map-sequence-id-507343

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  23. pYSD322

    Plasmid
    #253037
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-HA Frankenbody, MYC-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    igG 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253037-plasmid-map-sequence-id-507342

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  24. pYSD325

    Plasmid
    #253038
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Protein A ZZ domain, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Protein A 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253038-plasmid-map-sequence-id-507341

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  25. pYSD316

    Plasmid
    #253035
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, HA-tagged Aga1 deltaN181AA yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-GFP nanobody -Aga1
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253035-plasmid-map-sequence-id-507338

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  33. pEvolvR-mCherry-enCas9(D10A)-PolI5MΔ

    Plasmid
    #249068
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249068-plasmid-map-sequence-id-496320

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  34. pBA1751

    Plasmid
    #246066
    Purpose
    Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori
    Depositor
    Jennifer Doudna
    Article
    Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi
    Insert
    Panoptes locus
    Expression
    Bacterial
    Promoter
    pJEX promoter
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    246066-plasmid-map-sequence-id-491110

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  46. pBA1729

    Plasmid
    #246063
    Purpose
    Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori
    Depositor
    Jennifer Doudna
    Article
    Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi
    Insert
    Panoptes locus
    Expression
    Bacterial
    Promoter
    pTet promoter
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    246063-plasmid-map-sequence-id-490799

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Showing: 4401 - 4450 of 5049 results