We narrowed to 17,391 results for: puro

1. pLenti-FNLSHiFi-P2A-Puro

   Plasmid

   #136902

   Purpose

   Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFi
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R691A

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-HF1RA-P2A-Puro

   Plasmid

   #110853

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. OCT4 GFP puro Donor 3

   Plasmid

   #22211

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-VHL L188V-pBabe-puro

   Plasmid

   #19241

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Leucin 188 to Valin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. tet pLKO.1-shCSE1L v1 puro

   Plasmid

   #192345

   Purpose

   Lentiviral expression vector for an inducible shCSE1L v1

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   shCSE1L v1 (CSE1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. tet pLKO.1-shCSE1L v2 puro

   Plasmid

   #192346

   Purpose

   Lentiviral expression vector for an inducible shCSE1L v2)

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   shCSE1L v2 (CSE1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR

   Plasmid

   #190076

   Purpose

   express human ACE2 with selectable markers iRFP670 and puromycin resistance gene

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   none

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-Teton-puro-shFAK #1

   Plasmid

   #198757

   Purpose

   conditional knockdown of FAK

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

   Insert

   shFAK #1 (PTK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-Teton-puro-shFAK #2

   Plasmid

   #198758

   Purpose

   conditional knockdown of FAK

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

   Insert

   shFAK #2 (PTK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMT-puroR-p2a-HA-halo

    Plasmid

    #161979

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    puroR-p2a-HA-halo-GSlinker
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMY-MCS-P2A-PuroR-HA

    Plasmid

    #163352

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. plko-puromycin-shTRX1-259

    Plasmid

    #110921

    Purpose

    To knockdown Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Young et al Biochem Biophys Res Commun. 2010 Feb 12;

    Insert

    TRX1 (TXN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-puro-Flag-TRAF5

    Plasmid

    #102679

    Purpose

    retroviral expression plasmid for TRAF5

    Depositor

    Hasam Habelhah

    Article

    Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

    Insert

    TRAF5 (Traf5 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Puro-RalA-V23

    Plasmid

    #15252

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    v-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed 23rd amino acid to valine

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pBabe puro RalA (short) wt

    Plasmid

    #19723

    Depositor

    Channing Der

    Article

    Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

    Insert

    V-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NOTE: short splice variant Long Sp. Variant: MV…

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. HA-VHL Y98H-pBabe-puro

    Plasmid

    #19235

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Histidin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330(puro) Rosa26-H3F3B

    Plasmid

    #73131

    Purpose

    Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    spCas9
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

    Plasmid

    #105023

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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