We narrowed to 31,661 results for: uros

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pSB700 lenti gRNA Puro_zhang2.0

Plasmid

#167919

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB S28N

Plasmid

#19722

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

S28N for dominant negative function.

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1-dLIR

Plasmid

#74204

Purpose

Retroviral expression of GFP-NBR1 with aa 727-738 deletion

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 727-738

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
qTAG-N-Puro-sTagRFP-TUBA1B

Plasmid

#207769

Purpose

Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-TetOne-Puro-PFDN2

Plasmid

#202664

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

prefoldin subunit 2 (PFDN2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-WNK3

Plasmid

#222674

Purpose

endogenously tag WNK3 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

WNK3 (WNK3 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAuroraA GFP-AURKA-GFP

Plasmid

#157765

Purpose

Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

promoter CMV replaced by AurkA promoter

Promoter

AURKA minimal

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe HA-∆Raf1(S642A):ER* puro

Plasmid

#72572

Purpose

Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

Raf1 (RAF1 Human)

Use

Retroviral

Tags

ER* and HA

Expression

Mammalian

Mutation

amino acids 306-648, serine 642 mutated to alanine

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pTRE-T2-∆mIR-IRES-Puromycin

Plasmid

#105168

Purpose

Mammalian expression vector for expressing tandem cDNAs

Depositor

Michael E. Wright

Type

Empty backbone

Expression

Mammalian

Promoter

Tet/CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB Q72L

Plasmid

#19721

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

Q72L constitutively active, GTPase Deficient

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-SauriCas9-Puro

Plasmid

#135965

Purpose

Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pL6-H2B-iRFP720-puro

Plasmid

#189992

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 N200Q

Plasmid

#121451

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N200Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 pBabe puro

Plasmid

#13604

Depositor

Dean Sheppard

Insert

integrin alpha 9 (ITGA9 Human)

Use

Retroviral

Expression

Mammalian

Mutation

contains mature ITGA9 (aa30-1035 compared to NP_0…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only
plentiCRISPRv2-PURO-LYSET-gRNA1

Plasmid

#210489

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA1 (LYSET Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-PURO-LYSET-gRNA2

Plasmid

#210490

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA2 (LYSET Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro C-Raf 22W

Plasmid

#12593

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

N-term truncated mut of Raf-1 (RAF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

22W, N-terminally truncated

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe puro Myr HA PIK3CB

Plasmid

#12527

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele.

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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plex_307-3xflag-ccdb-puro

Plasmid

#201122

Purpose

pLEX_307 plasmid with N-terminal FLAG tag and puro marker

Depositor

Alejandro Chavez

Type

Empty backbone

Tags

FLAG

Expression

Mammalian

Promoter

EF-1α

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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