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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,308 results for: sequence

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Showing: 8951 - 8960 of 13308 results

  5. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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  7. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Article
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available Since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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  9. pPPED

    Plasmid
    #162468
    Purpose
    Destination Expression vector for dicot plant prime editing
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine based sequence o…
    Promoter
    2x35S
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162468-plasmid-map-sequence-id-350748

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  10. pSP6-RNAP

    Plasmid
    #48143
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase SP6
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    V314A (numbering based on NCBI Sequence ID: NP_85…
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48143-plasmid-map-sequence-id-373044

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Showing: 8951 - 8960 of 13308 results