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Showing: 321 - 360 of 360 results

  1. pNK3071

    Plasmid
    #219755
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219755-plasmid-map-sequence-id-450717

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  2. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219754-plasmid-map-sequence-id-458646

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  3. pFGL821

    Plasmid
    #58223
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    hygromycin B phosphotransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58223-plasmid-map-sequence-id-89704

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  4. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  5. pFGL821-8

    Plasmid
    #58224
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    hygromycin B phosphotransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58224-plasmid-map-sequence-id-89706

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  6. pFGL822

    Plasmid
    #58225
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    58225-plasmid-map-sequence-id-115839

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  7. pUG-amdSYM-mak10TR

    Plasmid
    #75433
    Purpose
    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae
    Depositor
    John McCusker
    Inserts
    MAK10TR
    ApR
    amdSYM
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminal 740 bp of MAK10 ORF
    Promoter
    Native promoter and PTEF
    Available since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75433-plasmid-map-sequence-id-142819

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  8. pFGL822-8

    Plasmid
    #58226
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58226-plasmid-map-sequence-id-89710

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  9. pAC574

    Plasmid
    #127975
    Purpose
    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    hygB (hygromycin resistance marker)
    Use
    Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127975-plasmid-map-sequence-id-254311

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  10. pAC575

    Plasmid
    #127976
    Purpose
    AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    ble (bleomycin resistance marker)
    Use
    Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127976-plasmid-map-sequence-id-254496

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  12. P307-FBP_6

    Plasmid
    #139697
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139697-plasmid-map-sequence-id-296513

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  13. P362-FBP_12

    Plasmid
    #139703
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139703-plasmid-map-sequence-id-296521

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  14. P336-FBP_11

    Plasmid
    #139702
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139702-plasmid-map-sequence-id-296517

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  15. P427-FBP_13

    Plasmid
    #139704
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139704-plasmid-map-sequence-id-296524

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  16. P305-FBP_4

    Plasmid
    #139695
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139695-plasmid-map-sequence-id-296191

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  17. P447-FBP_19

    Plasmid
    #139710
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139710-plasmid-map-sequence-id-296666

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  20. P303-FBP_2

    Plasmid
    #139693
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139693-plasmid-map-sequence-id-296189

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  21. pYFAC-ubi-Y-pyrG

    Plasmid
    #184497
    Purpose
    AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).
    Depositor
    Yit Heng Chooi
    Article
    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184497-plasmid-map-sequence-id-445302

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  22. P302-FBP_1

    Plasmid
    #139692
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    p35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139692-plasmid-map-sequence-id-296188

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  23. P313-FBP_8

    Plasmid
    #139699
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3H
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139699-plasmid-map-sequence-id-296197

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  24. P445-FBP_17

    Plasmid
    #139708
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139708-plasmid-map-sequence-id-296601

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  25. pYFAC-CH4

    Plasmid
    #168980
    Purpose
    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168980-plasmid-map-sequence-id-350001

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  26. pYFAC-CH2

    Plasmid
    #168978
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    168978-plasmid-map-sequence-id-353766

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  27. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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  28. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Tags
    Expression
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  29. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid
    #140202
    Purpose
    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Tags
    Expression
    Mutation
    G174D
    Promoter
    PelcA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140202-plasmid-map-sequence-id-310118

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  30. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid
    #140201
    Purpose
    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Cloning of crrna an…
    Tags
    dLbCas12a crRNA scaffold
    Expression
    Mutation
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140201-plasmid-map-sequence-id-310178

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  31. FB001

    Plasmid
    #119057
    Purpose
    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119057-plasmid-map-sequence-id-237151

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  33. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid
    #140197
    Purpose
    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140197-plasmid-map-sequence-id-309708

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  34. pYFAC-CH2-ubi-Y-pyrG

    Plasmid
    #221131
    Purpose
    Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    221131-plasmid-map-sequence-id-445434

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  35. pYFAC-CH2-ubi-M-pyrG

    Plasmid
    #221132
    Purpose
    Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    221132-plasmid-map-sequence-id-445432

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  37. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  38. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  39. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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