We narrowed to 54,294 results for: Ran

1. pCMV-Frankenbody-FLAG-SNAP

   Plasmid

   #175295

   Purpose

   Track mature FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with SNAP-tag
   

   Use

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLSSmOrange-mKate2 caspase-3 biosensor

   Plasmid

   #37132

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange-DEVD-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ601 - Peft-3 Mos1 transposase

   Plasmid

   #34874

   Purpose

   Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Mos1 transpoase
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SacI ML GFP Strand 11 Long

   Plasmid

   #164120

   Purpose

   Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Long
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Plasma membrane-targeted optoPLD

   Plasmid

   #140114

   Purpose

   Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-His6-Ran(Q69L)

   Plasmid

   #171563

   Purpose

   Encodes human Ran protein

   Depositor

   Yan Zeng

   Insert

   Ran (RAN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBactin-PACT-mOrange2-hCM1

   Plasmid

   #196868

   Purpose

   Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   PACT-mOrange2-hCM1 (Akap9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV ORANGE Dlg4-HaloTag KI

   Plasmid

   #139656

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SacI ML GFP Strand 11 Short

   Plasmid

   #164121

   Purpose

   Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Short
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMy-MouseCTCFbiotag-T2A-mOrange

    Plasmid

    #50564

    Purpose

    Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

    Depositor

    Rafael Casellas

    Article

    Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

    Inserts

    Ampicilin
    Mouse CTCF Exons
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDOE11 Lat52:mCherry transient

    Plasmid

    #124249

    Purpose

    Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

    Depositor

    Attila Fehér

    Article

    Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSP64-membrane-mCherry-LOV

    Plasmid

    #135599

    Purpose

    Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    membrane-mCherry-LOV
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    LOV and mCherry

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Plasma membrane-targeted optodPLD

    Plasmid

    #140061

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV ORANGE Gria1-HaloTag KI

    Plasmid

    #139655

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RHPMP133 (mOrange2-beta Actin)

    Plasmid

    #88837

    Purpose

    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2

    Depositor

    Ricardo Henriques

    Insert

    beta-Actin (ACTB Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RHPMP126 (mOrange2-alpha Tubulin)

    Plasmid

    #88835

    Purpose

    Localization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2

    Depositor

    Ricardo Henriques

    Insert

    alpha-tubulin (TUBA1B) (TUBA1B Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-DIO_EGFP-RandE3

    Plasmid

    #79872

    Purpose

    For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Mouse-Dysferlin-Transcript-Variant1

    Plasmid

    #60214

    Purpose

    Mouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

    Depositor

    Jain Foundation

    Insert

    Dysferlin (Dysf Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CamkII-MacQ-mOrange2

    Plasmid

    #48761

    Purpose

    sensor

    Depositor

    Mark Schnitzer

    Article

    Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

    Insert

    macQ-mOrange
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mac-D139Q

    Promoter

    CamKIIa

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Orange Nano-lantern/pcDNA3

    Plasmid

    #67661

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pBactin-mOrange2-NEDD1-gTBD

    Plasmid

    #196858

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD (NEDD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNH13 (pmyo-2::QuasAr::mOrange)

    Plasmid

    #130273

    Purpose

    Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr::mOrange
    

    Use

    Tags

    mOrange

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FCK-QuasAr2-mOrange2 eFRET

    Plasmid

    #59173

    Purpose

    Fluorescent membrane voltage sensor

    Depositor

    Adam Cohen

    Article

    Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

    Insert

    QuasAr2-mOrange2 eFRET
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-link-yoPSmOrange-Kan

    Plasmid

    #44951

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMTB2-Avi-Cerulean-RanGAP

    Plasmid

    #79878

    Purpose

    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSB1C3-J23110-B0034-mRFP1_Orange

    Plasmid

    #160444

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Orange
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Orange Nano-lantern/pRSETB

    Plasmid

    #67659

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

30. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid

    #68333

    Purpose

    Parvibaculum lavamentivorans Cas9

    Depositor

    Feng Zhang

    Insert

    PlCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p152 pWZL-EGFP RanBP10

    Plasmid

    #11098

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

32. Mouse-Dysferlin-Transcript-Variant2

    Plasmid

    #60215

    Purpose

    Mouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

    Depositor

    Jain Foundation

    Insert

    Dysferlin (Dysf Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. P-del (pTransluc-miR-145-2)

    Plasmid

    #21500

    Depositor

    Kenneth Kosik

    Article

    Xu et al Cell. 2009 May 15. 137(4):647-58.

    Insert

    0.9 kb 5' of miR-145 (MIR145 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV-EF1a-GFP RanSen

    Plasmid

    #184705

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. XE101 alpha transducin CS2+

    Plasmid

    #16681

    Depositor

    Randall Moon

    Article

    Slusarski et al Nature. 1997 Nov 27;390(6658):410-3. doi

    Insert

    alpha-transducin (GNAT1 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pFA6a-link-yoPSmOrange-SpHis5

    Plasmid

    #44856

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-hSyn-ChrimsonR-LSSmOrange

    Plasmid

    #149691

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15b-Fab1 (Francisella tularensis)

    Plasmid

    #61690

    Purpose

    Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

    Depositor

    Michael Johnson

    Article

    Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

    Insert

    enoyl-[acyl-carrier-protein] reductase (NADH)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pMTB2-RanGAP-Cerulean-Avi

    Plasmid

    #80068

    Purpose

    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Zebrafish biotagging

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAS1-tax-4P::his-24::mOrange

    Plasmid

    #124340

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    tax-4, mOrange (tax-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Francisella tularensis enoyl reductase

    Plasmid

    #231104

    Purpose

    Expresses FabI under T7 with HIS tag

    Depositor

    Josh Beckham

    Insert

    Enoyl-acyl carrier protein reductase
    

    Use

    Tags

    6x HIS, GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPB-cT3G-cERP2-RAN

    Plasmid

    #222533

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of RAN

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    RAN (RAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTRE3G-orangutan cGASdeltaN-GFP

    Plasmid

    #186846

    Purpose

    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Synthetic, Pongo abelii)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196855

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBactin-Nedd1-mOrange2-rCM1

    Plasmid

    #196861

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBactin-Nedd1-mOrange2-F75A

    Plasmid

    #196862

    Purpose

    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-F75A (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F75A in the CM1 domain

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKLV2-EF1a-GFP RanSen

    Plasmid

    #184697

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid

    #182680

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCIneo-Rluc-6xbsMS2-random_AC

    Plasmid

    #148431

    Purpose

    Mammalian Expression of 6xbsMS2-random

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    6xbsMS2-random
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. YIplac204-pOst1-ESCargo(Orange)

    Plasmid

    #140157

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge