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Plasmid#207121PurposeBacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purificationDepositorInsertHuman Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag (ABL1 Human)
UseTagsHiBiT Tag and His-tag with thrombin siteExpressionBacterialMutationCodon optmization for expression in BL21DE3PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40
Plasmid#233365PurposeCAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transductionDepositorInsertKinprola_PKA-mEGFP-NLS3X
UseAAVTagsmEGFP-NLS3XExpressionMutationPromoterCAGAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40
Plasmid#233364PurposeCAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transductionDepositorInsertNES-Kinprola_PKA-mTagBFP2
UseAAVTagsNES and mTagBFP2ExpressionMutationPromoterCAGAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40
Plasmid#233368PurposehSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_on-mEGFP
UseAAVTagsNES and mEGFPExpressionMutationPromoterhSyn1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40
Plasmid#233367PurposehSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseAAVTagsNES and mEGFPExpressionMutationPromoterhSyn1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP
Plasmid#233356PurposeCMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_off-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233353PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP
Plasmid#233354PurposeCMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP
Plasmid#233359PurposeCMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP
Plasmid#233360PurposeCMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP
Plasmid#233358PurposeCMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP
Plasmid#233355PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_on-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP
Plasmid#233362PurposeCMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP
Plasmid#233361PurposeCMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD
Plasmid#233363PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubuleDepositorInsertKinprola_PKA-mEGFP-MTBD
UseTagsmEGFP-MTBDExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP
Plasmid#233357PurposeCMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233369PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_PKA-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP
Plasmid#233370PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_on-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
NOLC1 nicking guide pDY2251
Plasmid#219861PurposeNicking sgRNA for NOLC1DepositorInsertNOLC1 nicking sgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin Ubl P37L
Plasmid#227366PurposeFor bacterial expression of mutated parkin Ubl.DepositorInsertParkin 1-76 P37L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only