We narrowed to 22,316 results for: Sele

1. pSLC-246

   Plasmid

   #73190

   Purpose

   Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   PrhaB-tse2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NsvBa

   Plasmid

   #100015

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

   Depositor

   David Clapham, Paul DeCaen

   Article

   DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

   Insert

   NsvBa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pR6Kg-Alpha1

   Plasmid

   #165862

   Purpose

   Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pR6Kg-Omega1

   Plasmid

   #165864

   Purpose

   Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3XP3-WhiteA2

   Plasmid

   #165875

   Purpose

   Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Eye Marker Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLC-239

   Plasmid

   #73193

   Purpose

   Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   PrhaB-yhaV (yhaV )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVD2

   Plasmid

   #165857

   Purpose

   universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGal4BD 1-147

   Plasmid

   #165819

   Purpose

   1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Gal4BD 1-147
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAL4AD 768-881

    Plasmid

    #165820

    Purpose

    768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GAL4AD 768-881
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Promoterless TP10

    Plasmid

    #165787

    Purpose

    Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Promoterless T10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGMR-WhiteA2

    Plasmid

    #165874

    Purpose

    Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-Duet1-His-TEV-mCherry-p62

    Plasmid

    #199780

    Purpose

    Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    6x His, TEV, mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-p62-Silent_Mutation_WT

    Plasmid

    #187986

    Purpose

    human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation for resistance to sip62 #5

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-AID

    Plasmid

    #61926

    Purpose

    Expression of human AID tagged with EGFP

    Depositor

    Jon Houseley

    Article

    Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

    Insert

    AICDA (AICDA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLC-238

    Plasmid

    #73192

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-chpB (chpB )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P[acman]-Alpha2

    Plasmid

    #165859

    Purpose

    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLC-241

    Plasmid

    #73194

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-mqsR (mqsR )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165887

    Purpose

    G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge