165,947 results

1. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

   Plasmid

   #223079

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMIA4.721-Keppel

   Plasmid

   #214444

   Purpose

   BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   2xFKBP12-Casp9
   

   Use

   Hdr donor

   Tags

   P2A-Bsd

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-KIF1B beta-FL

   Plasmid

   #79108

   Purpose

   Mammalian expression of GFP-KIF1B beta-FL

   Depositor

   Susanne Schlisio

   Article

   Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

   Insert

   GFP-KIF1B beta full length (KIF1B Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   exon 25 missing (natural splice varient)

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-FLAG-CAMK2D

   Plasmid

   #221687

   Purpose

   Mammalian expression of FLAG-tagged human CAMK2D

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   CAMK2D (CAMK2D Human)
   

   Expression

   Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX317-CELF4

   Plasmid

   #115445

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   CELF4 (CELF4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAF0690 Ef1a-Kp03_attB-BFP acceptor

   Plasmid

   #193473

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBUE411-B

   Plasmid

   #198234

   Purpose

   pPLTP::BBM cassette in pBUE411

   Depositor

   Andrea Gallavotti

   Insert

   pPLTP::BBM cassette
   

   Use

   CRISPR; Plant binary vector

   Expression

   Plant

   Promoter

   PLTP

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJMP1358

   Plasmid

   #119277

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_CDK4_p.R24C

   Plasmid

   #82175

   Purpose

   Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Gateway entry vector

   Mutation

   R24C

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5E_unc503

    Plasmid

    #64020

    Purpose

    5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
    

    Use

    Gateway

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge