We narrowed to 2,063 results for: ENDOG

1. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

   Plasmid

   #183609

   Purpose

   Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   Neo
   mKate2-3xNLS
   

   Use

   Mouse Targeting

   Tags

   3xNLS

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23-Fosprom

   Plasmid

   #188113

   Purpose

   Expresses luciferase under control of Fos promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

   Insert

   Fos promoter (Fos Mouse)
   

   Use

   Luciferase

   Promoter

   Fos endogenous promoter

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKnockinDonor-human-p16INK4A-P2A-mCherry

   Plasmid

   #137026

   Purpose

   donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

   Depositor

   Chunliang Li

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

   Insert

   p16INK4A-knockin-homology arm-P2A-mCherry
   

   Tags

   P2A-mCherry

   Expression

   Bacterial

   Promoter

   no promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNR52-sgTEF1

   Plasmid

   #46922

   Purpose

   Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgTEF1 promoter (TEF1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous TERT promoter

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUF1-dCas9-GCN5

   Plasmid

   #122509

   Purpose

   Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Insert

   dCas9-GCN5
   

   Use

   CRISPR

   Tags

   3*FLAG

   Mutation

   dCas9(D10A,H840A)

   Promoter

   PfHsp86

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TetON-dual-SunTag-Hygro

   Plasmid

   #121119

   Purpose

   Endogenous gene activation with the SunTag CRISPR activation system

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   dCas9-GCN4x10_scFv-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP777_SnoopCatcher-p65HSF1-CO

   Plasmid

   #211770

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-p65HSF1
   

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. RepairTemplate_NIPBL_mEGFP_mAID

   Plasmid

   #217664

   Purpose

   Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   NIPBL_mEGFP_mAID (NIPBL Human)
   

   Tags

   mAID and meGFP

   Expression

   Bacterial and Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP775_αGCN4-p65HSF1-CO

    Plasmid

    #211768

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-p65HSF1
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TetO-FUW-VdC9BV

    Plasmid

    #62195

    Purpose

    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

    Depositor

    Kam Leong

    Article

    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

    Insert

    VP64dCas9BFPVP64
    

    Use

    Lentiviral

    Tags

    Two VP64s tagged to dCas9 fused to BFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive) Cas9 (dCas9)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP786_SpyCatcher-EZH2-FL-CO

    Plasmid

    #211779

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-KRAB
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP778_Traptavidin-p65HSF1-CO

    Plasmid

    #211771

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-p65HSF1
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP776_SpyCatcher-p65HSF1-CO

    Plasmid

    #211769

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-p65HSF1
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP617_αGCN4-mCherry

    Plasmid

    #211785

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-DNMT3A
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLP783_SpyCatcher-DNMT3A-CO

    Plasmid

    #211776

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavudin-DNMT3A
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP788_Traptavidin-EZH2-FL-CO

    Plasmid

    #211781

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-EZH2
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HR-TERT-promotor

    Plasmid

    #71398

    Purpose

    Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Promoter

    Endogenous TERT promotor

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUF1-dCas9-Sir2a

    Plasmid

    #122510

    Purpose

    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-Sir2a
    

    Use

    CRISPR

    Tags

    3*FLAG

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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