We narrowed to 27,845 results for: UNK

1. pSD117A

   Plasmid

   #218141

   Purpose

   Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40
   

   Use

   Tags

   AviTag-6xHis-MBP-TEV-(GGGGS)2

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT7-EGFP-C1-HsXRN1-del1174-1649_AH

   Plasmid

   #148904

   Purpose

   Mammalian Expression of HsXRN1-del1174-1649

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

   Insert

   HsXRN1-del1174-1649 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-DmGIGYF_641-1574_AH

   Plasmid

   #148920

   Purpose

   Insect Expression of DmGIGYF_641-1574

   Depositor

   Elisa Izaurralde

   Article

   Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

   Insert

   DmGIGYF_641-1574 (Gyf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-DmGIGYF_1-640_AH

   Plasmid

   #148919

   Purpose

   Insect Expression of DmGIGYF_1-640

   Depositor

   Elisa Izaurralde

   Article

   Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

   Insert

   DmGIGYF_1-640 (Gyf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-Lyn-AktAR2

   Plasmid

   #175013

   Purpose

   Plasma membrane-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   Lyn-mCherry-Akt-STOPS
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-link-GFPIvy-SpHis5

   Plasmid

   #60783

   Purpose

   Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

   Depositor

   Linda Huang

   Article

   Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

   Insert

   GFPIvy
   

   Use

   Yeast integrating

   Tags

   Expression

   Mutation

   S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

   Promoter

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEG R2-iCreT2ALuc-L3 #BGV208

   Plasmid

   #48992

   Purpose

   Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Inserts

   cre recombinase
   Luciferase
   

   Use

   Luciferase; Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

   Plasmid

   #146503

   Purpose

   Insect Expression of DmDCP1_327-372

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   DmDCP1_327-372 (Dcp1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NET33 pmRFP-N2 (448)

   Plasmid

   #61979

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET33 (SCARA5 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pFlag-CMV2-Brd4 FEE-AAA

    Plasmid

    #21942

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1209-1362 only, FEE-AAA

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pFvl160

    Plasmid

    #64753

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmCup_C

    Plasmid

    #146041

    Purpose

    Insect Expression of DmCup

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmCup (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    P418S

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-EGFP-DmLSm4_A

    Plasmid

    #145883

    Purpose

    Insect Expression of DmLSm4

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    DmLSm4 (LSm-4 Fly)
    

    Use

    Tags

    V5-6xHis

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence of …

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFlag-CMV2-Brd4 R1337P

    Plasmid

    #21939

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1209-1362 only, R1337P

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pHL 1277

    Plasmid

    #53017

    Purpose

    AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    GFP
    mCherry
    

    Use

    Synthetic Biology

    Tags

    RBS(st7)

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pFlag-CMV2-Brd4 S1351R

    Plasmid

    #21941

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa1209-1362 only, S1351R

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pFlag-CMV2-Brd4 D1346N

    Plasmid

    #21940

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1209-1362 only, D1346N

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pHL 1278

    Plasmid

    #53018

    Purpose

    AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    mCherry
    GFP
    

    Use

    Synthetic Biology

    Tags

    RBS(st7)

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pT7-EGFP-C1-HsXRN1_1174-1649_AH

    Plasmid

    #148903

    Purpose

    Mammalian Expression of HsXRN1_1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1_1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHL 1405

    Plasmid

    #53010

    Purpose

    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains -56 to +141 relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pKV005

    Plasmid

    #64763

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tmem194 pmRFP-N2 (1389)

    Plasmid

    #62048

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem194 (TMEM194A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pVenus-N1 cyclin B1 L45A (1324)

    Plasmid

    #61851

    Purpose

    mammalian expression of cyclin B1 L45 A mutant fused to Venus

    Depositor

    Jonathon Pines

    Article

    Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pKV006

    Plasmid

    #64764

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHL718

    Plasmid

    #62826

    Purpose

    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    gfp
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ipex leader and ompC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pHL1855

    Plasmid

    #53041

    Purpose

    PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    tetR
    csrA
    

    Use

    Synthetic Biology

    Tags

    LVA, RBS(st3), and glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind and pLlac0-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pHL 1032

    Plasmid

    #52997

    Purpose

    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pPSmF

    Plasmid

    #226912

    Purpose

    Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPSmR

    Plasmid

    #226913

    Purpose

    Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pACYCDuet-1:ChrA-C63S-C66S

    Plasmid

    #225082

    Purpose

    Expresses precursor peptide ChrA-C63S/C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S/C66S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S/C66S

    Promoter

    T7

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACYCDuet-1:ChrA-C66S

    Plasmid

    #225081

    Purpose

    Expresses precursor peptide ChrA-C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C66S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C66S

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACYCDuet-1:ChrA-C63S

    Plasmid

    #225080

    Purpose

    Expresses precursor peptide ChrA-C63S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDFDuet-1:ChrH-ChrI

    Plasmid

    #225083

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSD118A

    Plasmid

    #218140

    Purpose

    Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD0_ZF2-3
    

    Use

    Tags

    AviTag-6xHis-MBP-TEV-(GGGGS)2

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSD068F

    Plasmid

    #218137

    Purpose

    Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD36-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSD113A

    Plasmid

    #218133

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJM154

    Plasmid

    #218121

    Purpose

    Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    FKBP12
    luxAB
    

    Use

    Luciferase

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH

    Plasmid

    #148936

    Purpose

    Insect Expression of DmGIGYF1_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1_641-1574 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH

    Plasmid

    #148937

    Purpose

    Insect Expression of DmGIGYF-F361AF367A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-F361AF367A (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH

    Plasmid

    #148938

    Purpose

    Insect Expression of DmGIGYF-W349A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-W349A (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge