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We narrowed to 14,587 results for: pET

Showing: 3561 - 3600 of 14587 results

  1. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid
    #101239
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    Fgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101239-plasmid-map-sequence-id-199088

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  2. pET15b/GgVcl 884-1066-ECFP

    Plasmid
    #46182
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Use
    Tags
    ECFP and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (tail domain)
    Promoter
    T7
    Available since
    July 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Use
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  12. pET-His6-Sumo-MBP-CfaN

    Plasmid
    #126016
    Purpose
    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)
    Depositor
    Thomas Muir
    Insert
    His5-Sumo-MBP-CfaN
    Use
    Tags
    5xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126016-plasmid-map-sequence-id-248770

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  16. Ana GvpC-SpyTag-His in pET28a

    Plasmid
    #85737
    Purpose
    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (fused to SpyTag and His-tagged)
    Use
    Tags
    His-tag and SpyTag
    Expression
    Bacterial
    Mutation
    Added an extra glycine after the methionine at th…
    Promoter
    T7
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    85737-plasmid-map-sequence-id-173841

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  20. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid
    #185791
    Purpose
    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-sfGFP(S45C)-ZdK1
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Ser to Cys mutation at start of GFP, residue 45 i…
    Promoter
    T7
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185791-plasmid-map-sequence-id-367545

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  26. pET-His6-Sumo-IntNtr-eSrt2A

    Plasmid
    #126015
    Purpose
    Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)
    Depositor
    Thomas Muir
    Insert
    His6-Sumo-IntNtr-eSrt2A
    Use
    Tags
    6xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126015-plasmid-map-sequence-id-248768

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  36. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  39. HOXA5 (human) HIS-tag pET

    Plasmid
    #8562
    Depositor
    Corey Largman
    Insert
    HOXA5 (HOXA5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    SacI site at amino acids 1-2 was used to clone in…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  40. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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Showing: 3561 - 3600 of 14587 results