We narrowed to 10,731 results for: Kars
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Plasmid#173042PurposeCustom vector for sgRNA library constructionDepositorInsertHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1950
Plasmid#205983PurposeGa0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DRDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTET2-lucCP-3MS2
Plasmid#198351PurposePart of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTRDepositorInsertFirefly Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRMPVneo.Yap.3093
Plasmid#59915PurposeTet on shRNA for conditional Yap1 knockdownDepositorInsertYap1 (Yap1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS1928
Plasmid#205967PurposeJAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)DepositorInsertCas8-HNH all proteins, with His-tagged Cas8
UseTags14xHis on Cas8ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
B270 + SPRTN sgSTOP
Plasmid#100718PurposeB270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOPDepositorInsertsgSTOP targeting SPRTN (cloned using BbsI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
UseTags3' extension derived from endogenous locus a…ExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-eIF4E
Plasmid#33252DepositorInserteIF4E (EIF4E Human)
UseRetroviralTagsuntaggedExpressionMammalianMutationunmodifiedPromoterSV40Available sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
TfR-moxGFP-DHFR
Plasmid#173012PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).DepositorInsertTfR-moxGFP-DHFR
UseTagsDHFR and moxGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS-CG-WT-Cer-VWF
Plasmid#124827PurposeExpresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.DepositorInsertVWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall charged residues are changed to S/Q except fo…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pHS1862
Plasmid#205975PurposeFLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas5 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK S6K (E389-deltaCT) neo
Plasmid#58516PurposeLentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseLentiviralTagsHAExpressionMammalianMutationT389E, stop codon at amino acid 402PromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG -YWAA-pGK-HYG
Plasmid#107381PurposeMammalian expression of FLAG-YWAADepositorInsertcereblon (CRBN Human)
UseTagsFLAGExpressionMammalianMutationCRBN-Y383A/W385APromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA
Plasmid#176538PurposeMammalian expression of nuclear localized AwaIscBDepositorInsertAwaIscB
UseTagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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ATP9A-Tol2
Plasmid#113385Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-luciferase
Plasmid#113354Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-mCherry-T2A-RPL22-1xV5
Plasmid#170324PurposeExpresses V5 and mCherry tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MESP1_P2A_Hygro_Barcode
Plasmid#120459PurposeBarcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMESP1 (MESP1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLE1_1
Plasmid#160762PurposeSuppress POLE1DepositorInsertshPOLE1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 K137R
Plasmid#118353PurposeThis plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.DepositorInserthGATA-1 (GATA1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK137RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-G6
Plasmid#194589PurposeBacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertG6 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mScarlet-gamma9
Plasmid#140097PurposeExpresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocationDepositorInsertGNGT2
UseTagsN-terminal mScarlet tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shJUND puro
Plasmid#58698PurposeLentiviral shRNA vector for knockdown of human JUNDDepositorInsertshJUND (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Csk-SH2
Plasmid#46420DepositorInsertCsk (CSK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 74-177PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;I848V)
Plasmid#33253DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and isoleucine…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTarget-RFP-GFP operon
Plasmid#192279PurposeRFP GFP fusion protein expressed under a constitutive promoterDepositorInsertRFP GFP
UseTagsExpressionBacterialMutationPromoterPlacIqAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PDZb-beta2(616-951)-mCherry
Plasmid#100736PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsPDZb and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-CRBN-pGK-HYG
Plasmid#107376PurposeLentiviral expression of myc-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLKO.1P POLD1_2
Plasmid#160792PurposeSuppress POLD1DepositorInsertshPOLD1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-smFP(myc)-KDEL
Plasmid#173021PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.DepositorInsert2xFKBP-smFP(myc)-KDEL
UseTagsFKBP and smFP(myc) non-fluorescentExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1518
Plasmid#205981PurposeFLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1514
Plasmid#205977PurposeFLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas8/Cse1 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1515
Plasmid#205978PurposeFLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas11/Cse2 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Ehmt1_2
Plasmid#36336DepositorInsertEhmt1 (EHMT1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLE1_2
Plasmid#160764PurposeSuppress POLE1DepositorInsertshPOLE1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-MYC.Mm.KAT5
Plasmid#184652PurposeRetroviral expression of mouse Kat5DepositorInsertKat5 (Kat5 Mouse)
UseRetroviralTagsMYCExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only