We narrowed to 11,576 results for: Kars
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Plasmid#169312PurposeConstitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent proteinDepositorInsertmiR-ctrl
UseLentiviralTagsTagBFP2PromoterSFFVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-EBFP2-GMRWhite
Plasmid#165921PurposeBlasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-sfGFP-MiniWhite
Plasmid#165922PurposeBlasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-EBFP2-MiniWhite
Plasmid#165924PurposeBlasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-EBFP2-MiniWhite
Plasmid#165912PurposeBlasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG418R-MCS-LexA-VP16-MiniWhite
Plasmid#165894PurposeG418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.DepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_K90E-IRES_hygro
Plasmid#166407PurposeExpress hPOT1 K90E in mammalian cellsDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-mCherry-GMRWhite
Plasmid#165908PurposeBlasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-mCherry-GMRWhite
Plasmid#165920PurposeBlasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-sfGFP-MiniWhite
Plasmid#165916PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyAvailable SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-mCherry-MiniWhite
Plasmid#165923PurposeBlasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_dead_mt
Plasmid#167270PurposeOverexpression of the dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialMutationC145APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
TagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1A CbbL-
Plasmid#162693Purposep1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and PrkDepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialMutationCbbL K194M (inactive rubisco)PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-PIGA
Plasmid#153958PurposeUsed for EGxxFP assay with PIGA intron 5 and exon 6 sequencesDepositorInsertPIGA (a 401-bp fragment from intron 5 and exon 6)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA852 - mec-4p::FynY531F
Plasmid#139209PurposeExpresses activated Fyn kinase in mec-4 neuronsDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOG_D1
Plasmid#139112Purposeexpress first domain (1-250) of TOGDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_RtoK
Plasmid#139120Purposeexpress hnRNPA2 LC where all R are KDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_NtoS
Plasmid#139121Purposeexpress hnRNPA2 with all N changed to SDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_5E
Plasmid#139122Purposeexpress hnRNPA2 with 5 serine changed to glutamateDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_12E
Plasmid#139123Purposeexpress hnRNPA2 with all serine change to glutamateDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPF_PLD_YtoS
Plasmid#139124Purposeexpress hnRNPF PLD with all Y changed to SDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPF_PLD_StoA
Plasmid#139125Purposeexpress hnRNPF PLD with all S changed to ADepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT139_GalL_nCDA1-VRERBE3
Plasmid#145102PurposeExpressing base editor nCDA1-VRERBE3 in yeast cellsDepositorInsertnCDA1-VRERBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT92_GalL_nCDA1Δ188-BE3
Plasmid#145052PurposeExpresses nCDA1Δ188-BE3 in yeast cellsDepositorInsertnCDA1Δ188-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-188AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT90_GalL_nCDA1Δ190-BE3
Plasmid#145050PurposeExpresses nCDA1Δ190-BE3 in yeast cellsDepositorInsertnCDA1Δ190-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-190AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT85_GalL_nCDA1Δ193-BE3
Plasmid#145048PurposeExpresses nCDA1Δ193-BE3 in yeast cellsDepositorInsertnCDA1Δ193-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-193AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT84_GalL_nCDA1Δ194-BE3
Plasmid#145047PurposeExpresses nCDA1Δ194-BE3 in yeast cellsDepositorInsertnCDA1Δ194-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-194AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT78_GalL_nCDA1Δ195-BE3
Plasmid#145044PurposeExpresses nCDA1Δ195-BE3 in yeast cellsDepositorInsertnCDA1Δ195-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-195AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LMX1B-gRNA-C-del
Plasmid#131516PurposegRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-delDepositorInsertLMX1B-gRNA-C-del
UseCRISPRExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 D353A
Plasmid#133016PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationD353APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 C351A
Plasmid#133014PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationC351APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b K294R
Plasmid#118357PurposeThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS2
Plasmid#113091PurposeLuciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p bindingDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationnonePromoterPGKAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS1
Plasmid#113090PurposeLuciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding siteDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationnonePromoterPGKAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
TagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-AVITEV-GFP
Plasmid#74052Purposeretroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInsertAVITEV-GFP
UseRetroviralTagsAVI-TEV-tag N-terminal to the GFPExpressionMammalianPromoterpMSCV-LTRsAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-D92N
Plasmid#58412PurposeExpresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationApartate 92 to AsparaginePromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24,88,104A
Plasmid#58406PurposeExpresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysines 24, 88, and 104 to AlaninePromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-N2A
Plasmid#58410PurposeExpresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationAsparagine 2 to AlaninePromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-H136T
Plasmid#58413PurposeExpresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationHistidine 236 to ThreoninePromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24,83,104A
Plasmid#58407PurposeExpresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysines 24, 83, and 104 to AlaninePromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-S38N
Plasmid#58411PurposeExpresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationSerine 38 to AsparaginePromoterCMV IEAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24,83,88A
Plasmid#58404PurposeExpresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysines 24, 83, and 88 to AlaninePromoterCMV IEAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K88A
Plasmid#58402PurposeExpresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysine 88 to AlaninePromoterCMV IEAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only