We narrowed to 5,263 results for: 290

1. pcDNA3.1-mCherry-CyrCR1

   Plasmid

   #128768

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM2-hPMCA4b

   Plasmid

   #47759

   Purpose

   mammalian expression of human PMCA4b

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

   Insert

   PMCA4b (ATP2B4 Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-28a-YTH domain of YTHDF3

   Plasmid

   #228777

   Purpose

   Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF3 (YTHDF3 Human)
   

   Tags

   6*His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hHSF1 K80R

   Plasmid

   #118350

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to R.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2/Capsid

   Plasmid

   #120816

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   Capsid ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/ATP10D(E215Q)-HA

   Plasmid

   #209248

   Purpose

   Mammalian expression of ATP10D mutant E215Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10D (ATP10D Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E215Q

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2/Envelope

   Plasmid

   #120821

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   Envelope ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBH-UAS-secA5-YFP

   Plasmid

   #32359

   Purpose

   Live imaging of apoptotic cells in zebrafish

   Depositor

   Randall Peterson

   Article

   van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

   Insert

   secreted human annexin 5 (ANXA5 Human)
   

   Use

   Zebrafish expression

   Promoter

   UAS

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-Ngn2

    Plasmid

    #32928

    Purpose

    Retroviral expression of mouse Ngn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-5X1 Ash2L full

    Plasmid

    #15549

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid

    #129383

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

    Depositor

    Silvestro Conticello

    Article

    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

    Insert

    mCherry-ApoB-Tag-BFP
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SAC1delta452-587-FKBP-mCherry

    Plasmid

    #108131

    Purpose

    Recruitable inactive SAC1 (linker deletion)

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-451):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Expression

    Mammalian

    Mutation

    SACM1L(1-451)

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. phGR-SB

    Plasmid

    #133409

    Purpose

    overexpression of a human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid

    #189871

    Purpose

    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK1 (NEK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSALECTNK-TEM1(S70A,D179G)/csTEM1

    Plasmid

    #81163

    Purpose

    pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Tags

    Codon swapped TEM-1 from H26 to W290 and His6x

    Expression

    Bacterial

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    lac promoter

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2/pr-Membrane

    Plasmid

    #120817

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    pr-Membrane ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL-SFFV.Reporter.RFP657.PAC

    Plasmid

    #61395

    Purpose

    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-T3-CjCAS9-pA

    Plasmid

    #131543

    Purpose

    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    FLAG-SV40NLS, SV40NLS, and polyA

    Expression

    Mammalian

    Mutation

    Human codon-optimized

    Promoter

    CAG, T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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