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pEAHISPSVORF131

Plasmid

#97022

Purpose

Expressing Pyrobaculum spherical virus orf131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 131

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4511

Plasmid

#97015

Purpose

Expressing Pseudomonas aeruginosa orf PA4511 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P. aeruginosa orf PA4511

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMIH-TOMM20-Halo

Plasmid

#111626

Purpose

Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TOMM20 (Tomm20 Mouse)

Use

Retroviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF-FH-TAZ S89A-ires-blast

Plasmid

#52084

Purpose

Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

TAZ S89A (WWTR1 Human)

Use

Lentiviral

Tags

FLAG and His

Expression

Mammalian

Mutation

S89A

Promoter

EF1a

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

Plasmid

#65417

Purpose

AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Has Service

AAV8

Insert

hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Expression

Mutation

Promoter

hSyn

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-YTHDC1-FL-mCherry-Cry2

Plasmid

#177124

Purpose

Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTHDC1 Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

Plasmid

#65418

Purpose

AAV packaging vector for CamKIIa driven KOR DREADD expression

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Insert

CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Expression

Mutation

Promoter

CaMKIIa

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAX

Plasmid

#111628

Purpose

Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EF-FH-TAZ-ires-blast

Plasmid

#52083

Purpose

Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

FLAG and His

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAK

Plasmid

#111627

Purpose

Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

BAK (Bak1 Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#129418

Purpose

Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgRAB18.N (RAB18 Human)

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-SomaGCaMP7

Plasmid

#182941

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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elavl3:SomaGCaMP7f

Plasmid

#158760

Purpose

Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

HuC promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Soma-jGCaMP8f

Plasmid

#182943

Purpose

Cell body-targeted jGCaMP8f, under synapsin promoter.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-jGCaMP8f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-BlastR [M1G]

Plasmid

#171805

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-BlastR

Use

Gene tagging

Tags

Expression

Mutation

BlastR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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