We narrowed to 9,259 results for: pho

1. pcDNA5 FRT/TO Plk4 ∆24-mCherry

   Plasmid

   #80271

   Purpose

   mammalian expression plasmid for Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Use

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lyn11-CIBN-GFP

   Plasmid

   #79572

   Purpose

   expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   Lyn11
   

   Use

   Tags

   CIBN (CRY2-binding domain) and EGFP

   Expression

   Mammalian

   Mutation

   G8R (please see depositor comments below)

   Promoter

   CMV

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

4. AKARdual

   Plasmid

   #99869

   Purpose

   Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   AKAR4
   

   Use

   Tags

   LSSmOrange and mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Use

   Tags

   24×Broccoli

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGATA-1 WT

   Plasmid

   #118352

   Purpose

   This plasmid expresses human GATA-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX304-mNeonGreen

   Plasmid

   #162034

   Purpose

   Tagged vector control

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Neon Green

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-Flag-Timeless

   Plasmid

   #23119

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Timeless (TIMELESS Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pACE-GIRK4-mC-S

   Plasmid

   #172427

   Purpose

   Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   mCherry Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKD-SepRS-EFSep

    Plasmid

    #34623

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Inserts

    SepRS (MMP0688 Methanocaldococcuc maripaludis)
    EF-Sep (tufB E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac and Ptac (from SepRS- operon like)

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2XMyc-LRRK2-Y1699C

    Plasmid

    #25364

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Tyrosine 1699 mutated to Cysteine

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-N1-CaMKKbeta

    Plasmid

    #32453

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tag5Amyc-GSK3b KD

    Plasmid

    #16262

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Kinase dead: K85A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Akt-DD

    Plasmid

    #39536

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt (Akt1 Mouse)
    

    Use

    Tags

    6G linker, EGFP, and HA

    Expression

    Mammalian

    Mutation

    DD = T308D and S473D

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C3-hYAP2

    Plasmid

    #17844

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-Daxx/pRK5

    Plasmid

    #52023

    Purpose

    Expresses HA-Daxx in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

    Insert

    HA-Daxx (DAXX Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-myc-hIFITM3-Y20A

    Plasmid

    #58462

    Purpose

    Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 to alanine

    Promoter

    CMV IE

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pJW6.01

    Plasmid

    #62882

    Purpose

    Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    GFP::MEG-3
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    meg-3

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 MDM2 S166D S186D

    Plasmid

    #16236

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser166 and Ser186 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-CMV-cGASΔN-HA

    Plasmid

    #130912

    Purpose

    Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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