We narrowed to 15,034 results for: pET

1. pET28b-GFP-tglG

   Plasmid

   #134679

   Purpose

   Expresses His6-GFP-TglG fusion in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglG
   

   Use

   Tags

   His6-GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSHDY*in_PpetE:BCD2:AgBIS:Flag

   Plasmid

   #133971

   Purpose

   Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.

   Depositor

   Pia Lindberg

   Article

   Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

   Insert

   AgBIS:Flag
   

   Use

   Synthetic Biology

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   inserted gene is codon optimized for Synechocyst…

   Promoter

   PpetE:BCD2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSHDY*in_PpetE:BCD2:MrBBS:Strep

   Plasmid

   #133973

   Purpose

   Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.

   Depositor

   Pia Lindberg

   Article

   Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

   Insert

   MrBBS:StrepII
   

   Use

   Synthetic Biology

   Tags

   StrepII

   Expression

   Bacterial

   Mutation

   inserted gene is codon optimized for Synechocyst…

   Promoter

   PpetE:BCD2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a SznF E281A

   Plasmid

   #139591

   Purpose

   expresses SznF E281A

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF E281A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 281 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a SznF H225A

   Plasmid

   #139590

   Purpose

   expresses SznF H225A

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H225A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed histidine 225 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-SznF E215A

   Plasmid

   #139565

   Purpose

   expresses SznF E215A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF E215A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-DDX43-NT-G46D

   Plasmid

   #134588

   Purpose

   Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G154D)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-DDX43-FL-G84D

   Plasmid

   #134577

   Purpose

   Express human DDX43 mutant (full-length with G84D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-G84D (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G84D)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET15b-GreBdm-D41N

    Plasmid

    #129691

    Purpose

    express E. coli GreB E82C/C68S/D41N

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S D41N (greB E. coli)
    

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJUMP18-RBS-pET_R

    Plasmid

    #127013

    Purpose

    Basic Part R- RBS; pET vector Ribosome Binding Site.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part RBS-pET_R
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK-petD 3'UTR

    Plasmid

    #123571

    Purpose

    MoChlo kit

    Depositor

    C. Neal Stewart, Scott Lenaghan

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    petD 3'UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28B-PU.1-ETS-Loop

    Plasmid

    #124551

    Purpose

    Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 216 to 223 replaced with corresponding s…

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-SOMA-T679A

    Plasmid

    #118936

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21d-colEdes7/Imdes7

    Plasmid

    #107115

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes7/Imdes7
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21d-colEdes8/Imdes8

    Plasmid

    #107116

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes8/Imdes8
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21d-colEdes9/Imdes9

    Plasmid

    #107117

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes9/Imdes9
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21d-colEdes10/Imdes10

    Plasmid

    #107118

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes10/Imdes10
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21d-colEdes11/Imdes11

    Plasmid

    #107119

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes11/Imdes11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21d-colEdes12/Imdes12

    Plasmid

    #107120

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes12/Imdes12
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21d-colEdes13/Imdes13

    Plasmid

    #107121

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des13

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes13/Imdes13
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21d-colEdes14/Imdes14

    Plasmid

    #107122

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des14

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes14/Imdes14
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21d-colEdes15/Imdes15

    Plasmid

    #107123

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des15

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes15/Imdes15
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21d-colEdes16/Imdes16

    Plasmid

    #107124

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes16/Imdes16
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21d-colEdes17/Imdes17

    Plasmid

    #107125

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes17/Imdes17
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21d-colEdes18/Imdes18

    Plasmid

    #107126

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes18/Imdes18
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge