We narrowed to 81,735 results for: TRI

Showing: 4501 - 4550 of 81735 results

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L1_UBE2:eG-Lt-Ck4

Plasmid

#136130

Purpose

L1 in position 4, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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PROM_MpNOP1

Plasmid

#136117

Purpose

PROM for cloning with 5UTR_MpNOP1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_MpNOP1

Plasmid

#136119

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck3

Plasmid

#136128

Purpose

L1 in position 3, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:eG-Lt-Ck3

Plasmid

#136129

Purpose

L1 in position 3, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_eGFP-HDEL

Plasmid

#136097

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-Lti6b

Plasmid

#136099

Purpose

Plasma Membrane targeting protein for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAla_Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-mVenus

Plasmid

#136101

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_mVenus

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-N7

Plasmid

#136102

Purpose

Nuclear targeting peptide for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSer4xAla_N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpNOP1

Plasmid

#136103

Purpose

5UTR for cloning with PROM_MpNOP1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mTurquoise2-HDEL

Plasmid

#136105

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mTurquoise2(noATG,noStop)-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mVenus-HDEL

Plasmid

#136107

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mVenus(noATG,noStop)-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_NLS-SV40

Plasmid

#136108

Purpose

CTAG Nuclear localiation signal SV40 for fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_NLS-SV40

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsB_spacer

Plasmid

#136076

Purpose

Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mTurquoise2-linker

Plasmid

#136090

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mVenus-linker

Plasmid

#136092

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mVenus(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_TP-MpSIG2

Plasmid

#136093

Purpose

Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

TP-MpSIG2, Mapoly0214s0004

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsA_spacer

Plasmid

#136075

Purpose

Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Tags

6xHis

Expression

Yeast

Promoter

MET25

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-ChPylTMSK

Plasmid

#163915

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

Plasmid

#139963

Purpose

Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNAM-B1

Plasmid

#154056

Purpose

Level 0 Golden Gate vector, contains domesticated NAM-B1

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

TaNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Synonymous mutation at base 3257, in exon 3, to r…

Promoter

N/A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BufAI-2

Plasmid

#128651

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UBQ10:sXVE:ER-S11-GFP1–9

Plasmid

#125666

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-S11-GFP1–9

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIII-Neo-AM

Plasmid

#153427

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW4.34

Plasmid

#131370

Purpose

expression of GST:MEG-4 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-4 (C36C9.1 Nematode)

Expression

Bacterial

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p1 AO7R

Plasmid

#137842

Purpose

GST tagged AO7 encoding aa 126-216 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PX330_ZBTB7B_2

Plasmid

#135747

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PX330_ZBTB7B_1

Plasmid

#135746

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW4.33

Plasmid

#131369

Purpose

expression of GST:MEG-3 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-3 (meg-3 Nematode)

Expression

Bacterial

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
INPP5A / type I IP3 5-phosphatase from dog thyroid

Plasmid

#131767

Purpose

Expression in bacteria or COS-7 cells

Depositor

Christophe Erneux

Article

Verjans et al Biochem J. 1994 May 15;300 ( Pt 1):85-90

Insert

INPP5A

Expression

Bacterial

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQlinkH-YbATP-grasp

Plasmid

#129639

Purpose

Expression plasmid of ATP-grasp from Y. bercovieri

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbATP-grasp

Tags

His6-tag

Expression

Bacterial

Promoter

pQE

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.31

Plasmid

#131371

Purpose

expression of GST:MEG-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-1 (meg-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG LacZ

Plasmid

#83093

Purpose

Gateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415TEF-NQYFP

Plasmid

#99554

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3_zfh1_CP-candidate_luc+

Plasmid

#86391

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only
pUAST attB BmOr2 (Y464A)

Plasmid

#73927

Purpose

UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

BmOr2 (Y464A)

Expression

Insect

Mutation

changed 464 Tyrosine to Alanine

Promoter

hsp70

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK359

Plasmid

#71570

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX1ZFCNGA5

Plasmid

#69859

Purpose

GST tagged protein for antibody production screening

Depositor

Gary Matthews

Article

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

GST

Expression

Bacterial

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD125 GFP:POP1

Plasmid

#71387

Purpose

localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

POP1 (POP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
pJB051 (mEos2-ZapA)

Plasmid

#72650

Purpose

Inducible expression of mEos2-ZapA in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

mEos2-ZapA

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK286

Plasmid

#71567

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

formyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK358

Plasmid

#71569

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 81,735 results for: TRI