We narrowed to 5,538 results for: pcas

1. pCAGGS-MTS(S34A)-EGFP-V5

   Plasmid

   #200932

   Purpose

   expresses MTS(S34A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S34A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS--MTS(S43A)-EGFP-V5

   Plasmid

   #200933

   Purpose

   expresses MTS(S43A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S43A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-MTS(S17A)-TFAM-V5

   Plasmid

   #203668

   Purpose

   expresses MTS(S17A)-TFAM-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   Transcription Factor A, Mitochondrial (TFAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S17A

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-MTS(S43A)-TFAM-V5

   Plasmid

   #203669

   Purpose

   expresses MTS(S43A)-TFAM-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   Transcription Factor A, Mitochondrial (TFAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S43A

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS-FLAG-BubR1-D911A

   Plasmid

   #200815

   Purpose

   expresses BubR1-D911A in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Aspartic acid 911 to Alanine

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-MTS(S6A)-EGFP-V5

   Plasmid

   #200817

   Purpose

   expresses MTS(S6A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   Changed Serine 6 to Alanine in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-MTS(S12A)-EGFP-V5

   Plasmid

   #200818

   Purpose

   expresses MTS(S12A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S12A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_mCherry-ATG13 HORMA (1-191aa)

   Plasmid

   #223759

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   ATG13 HORMA (ATG13 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa 1-191 only

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG/VPS15(R36D)-TSF

   Plasmid

   #232913

   Purpose

   Mammalian Expression

   Depositor

   James Hurley

   Insert

   VPS15 (PIK3R4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R36D

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG/VPS34(W885D/Y884D)

    Plasmid

    #232918

    Purpose

    Mammalian Expression

    Depositor

    James Hurley

    Insert

    VPS34 (PIK3C3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W885D/Y884D

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-GluA2-Fab-HC-Halo

    Plasmid

    #230061

    Purpose

    Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 Fab heavy chain fragment
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-U6-MYC-Guide-hSpCas9

    Plasmid

    #228574

    Purpose

    Expression of human MYC gRNA; CRISPR mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    MYC gRNA (MYC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA528 IST1 (322-366)

    Plasmid

    #193038

    Purpose

    Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 322-366

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG CAPN7 V18D-OSF

    Plasmid

    #193042

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    V18D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG CAPN7 L61D-OSF

    Plasmid

    #193043

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    L61D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG CAPN7 F98D-OSF

    Plasmid

    #193044

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    F98D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 CHMP1A 140-196

    Plasmid

    #104593

    Purpose

    Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1A residues 140-196 (CHMP1A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 CHMP2A 152-222

    Plasmid

    #104594

    Purpose

    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A residues 152-222 (CHMP2A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge