We narrowed to 33,255 results for: IND

1. HA-VHL Y112N-pBabe-puro

   Plasmid

   #19238

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 112 to Asparagin

   Promoter

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-VHL Y112H-pBabe-puro

   Plasmid

   #19237

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 112 to Histidin

   Promoter

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMGS59(AID-ARF-P2A-Hygromycin)

   Plasmid

   #138174

   Purpose

   An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

   Insert

   AID-ARF-P2A-Hygromycin
   

   Use

   Tags

   AID-ARF clamp degron tag

   Expression

   Mutation

   Promoter

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-STITCH

   Plasmid

   #129535

   Purpose

   STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   STITCH
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ScFv-2ERT2-V

   Plasmid

   #120553

   Purpose

   3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, sfGFP, GB1, ERT2, VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sg-tdTomato90/816

   Plasmid

   #179918

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-NES-ZapCY2

   Plasmid

   #59016

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

   Insert

   NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. HA-VHL L188V-pBabe-puro

   Plasmid

   #19241

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Leucin 188 to Valin

   Promoter

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCASP-SptP120-TD-HilA

   Plasmid

   #153330

   Purpose

   Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-HA4-7c12 Tandem Monobody
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmirGLO-Smad4-1719

    Plasmid

    #49383

    Purpose

    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

    Depositor

    Martine Roussel

    Article

    Murphy et al Cancer Res. 2013 Oct 21.

    Insert

    Smad family member 4 (SMAD4 Mouse, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Human phosphoglycerate kinase (PGK) promoter

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-4T1-Claspin-N

    Plasmid

    #31623

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    aa 1-679 of N-term

    Promoter

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

12. HA-VHL Y98H-pBabe-puro

    Plasmid

    #19235

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Histidin

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCASP-SptP167-3G124-HilA

    Plasmid

    #153322

    Purpose

    Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP167-3G124 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-VHL 1-115-pBabe-puro

    Plasmid

    #19243

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 116 - 213 (end)

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GAL 103Q+ProCFPp303

    Plasmid

    #15575

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCASP-SptP120-3m160-HilA

    Plasmid

    #153325

    Purpose

    Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3m160 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2xFKBP-myc

    Plasmid

    #20212

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFKBP (FKBP1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3 Ub-UBB+1

    Plasmid

    #23967

    Depositor

    Nico Dantuma

    Article

    Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

    Insert

    Ubiquitin B +1 transcript (UBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFS485

    Plasmid

    #89348

    Purpose

    Leu1 integrating plasmid with cdc25 genomic fragment

    Depositor

    Nick Rhind

    Article

    Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

    Insert

    cdc25 (cdc25 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5A0-T

    Plasmid

    #92016

    Purpose

    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    J23102 Promoter (from Anderson promoter library) …

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Vh-HA-2xFKBP

    Plasmid

    #20221

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFKBP (FKBP1A Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pLN431 (FuGW-G8p-mKate2-1Pe )

    Plasmid

    #105186

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-mKate2-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-EF1a-V5-Vrk2

    Plasmid

    #120240

    Purpose

    Expresses V5-tagged Vrk2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Vrk2 (Vrk2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Desmin/pAS2-1

    Plasmid

    #97407

    Purpose

    Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    Desmin (DES Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. GAL FLAG46QDProCFPp303

    Plasmid

    #15585

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

    Plasmid

    #81206

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GPD 25QDProGFP p416

    Plasmid

    #15568

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pCASP-SptP120-E3_5-HilA

    Plasmid

    #153326

    Purpose

    Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-E3_5 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCALNL-eGFP-NT1-6-gix-6-NT1

    Plasmid

    #81204

    Purpose

    contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NmMetQ N238A

    Plasmid

    #129648

    Purpose

    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence

    Depositor

    Douglas Rees

    Article

    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

    Insert

    metQ Gene
    

    Use

    Tags

    10x Histidine Tag, Enterokinase, and pelB signal …

    Expression

    Bacterial

    Mutation

    Changed Asparagine 238 to Alanine

    Promoter

    T7 Promoter

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

    Plasmid

    #105182

    Purpose

    Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(E2F1)p-ECFP (Cerulean) sponge control
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCASP-SptP120-K27-HilA

    Plasmid

    #153328

    Purpose

    Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K27 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. spa-GCaMP6f

    Plasmid

    #67558

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    CMV

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

34. GPD 104QDProGFP p416

    Plasmid

    #15569

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 104Q Delta Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

35. AAV-hSyn-Lyn-Y-GECO1

    Plasmid

    #58200

    Purpose

    Express the membrane targeted Y-GECO1 in neurons

    Depositor

    Robert Campbell

    Article

    Zhao et al Integr Biol (Camb). 2014 May 19.

    Insert

    Y-GECO1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GAL FLAG72QDProCFPp303

    Plasmid

    #15586

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pGEX2TK-YAP-WW1-RRK

    Plasmid

    #104306

    Purpose

    bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated

    Depositor

    Marius Sudol

    Insert

    YAP WW1 domain (YAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…

    Promoter

    tac

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p5B3-T

    Plasmid

    #92021

    Purpose

    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAVS1 Insulator ObLiGaRe/EPB71

    Plasmid

    #90018

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by insulator and inverted ZFN binding sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTorPE-GEX-GECO1

    Plasmid

    #32464

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge